In this work, we identified a nucleolar protein, HEATR1 as an important player of ribosome biogenesis. In the absence of this protein, ribosome biogenesis stress is activated, characterized by the elevated level of p53, cell cycle arrest and structural changes of the nucleolus. Furthermore, HEATR1 seems to be involved in the early steps of ribosome biogenesis, since it possesses similar localization signal to Pol I and its ablation completely abolishes rRNA transcription. Besides its function in ribosome biosynthesis, HEATR1 may be required for DNA damage signaling as well, evidenced by DNA damage-dependent phosphorylation of this protein. Overall, our work presents HEATR1, a novel player in ribosome biogenesis and DNA damage response.
Anotace v angličtině
In this work, we identified a nucleolar protein, HEATR1 as an important player of ribosome biogenesis. In the absence of this protein, ribosome biogenesis stress is activated, characterized by the elevated level of p53, cell cycle arrest and structural changes of the nucleolus. Furthermore, HEATR1 seems to be involved in the early steps of ribosome biogenesis, since it possesses similar localization signal to Pol I and its ablation completely abolishes rRNA transcription. Besides its function in ribosome biosynthesis, HEATR1 may be required for DNA damage signaling as well, evidenced by DNA damage-dependent phosphorylation of this protein. Overall, our work presents HEATR1, a novel player in ribosome biogenesis and DNA damage response.
Klíčová slova
Ribosome biogenesis, nucleolus, HEATR1, p53, cancer, DNA damage response
Klíčová slova v angličtině
Ribosome biogenesis, nucleolus, HEATR1, p53, cancer, DNA damage response
Rozsah průvodní práce
-
Jazyk
CZ
Anotace
In this work, we identified a nucleolar protein, HEATR1 as an important player of ribosome biogenesis. In the absence of this protein, ribosome biogenesis stress is activated, characterized by the elevated level of p53, cell cycle arrest and structural changes of the nucleolus. Furthermore, HEATR1 seems to be involved in the early steps of ribosome biogenesis, since it possesses similar localization signal to Pol I and its ablation completely abolishes rRNA transcription. Besides its function in ribosome biosynthesis, HEATR1 may be required for DNA damage signaling as well, evidenced by DNA damage-dependent phosphorylation of this protein. Overall, our work presents HEATR1, a novel player in ribosome biogenesis and DNA damage response.
Anotace v angličtině
In this work, we identified a nucleolar protein, HEATR1 as an important player of ribosome biogenesis. In the absence of this protein, ribosome biogenesis stress is activated, characterized by the elevated level of p53, cell cycle arrest and structural changes of the nucleolus. Furthermore, HEATR1 seems to be involved in the early steps of ribosome biogenesis, since it possesses similar localization signal to Pol I and its ablation completely abolishes rRNA transcription. Besides its function in ribosome biosynthesis, HEATR1 may be required for DNA damage signaling as well, evidenced by DNA damage-dependent phosphorylation of this protein. Overall, our work presents HEATR1, a novel player in ribosome biogenesis and DNA damage response.
Klíčová slova
Ribosome biogenesis, nucleolus, HEATR1, p53, cancer, DNA damage response
Klíčová slova v angličtině
Ribosome biogenesis, nucleolus, HEATR1, p53, cancer, DNA damage response