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Course info
ACH / PHS
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Course description
Department/Unit / Abbreviation
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ACH
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PHS
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Academic Year
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2024/2025
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Academic Year
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2024/2025
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Title
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Mass Spectrometry
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Form of course completion
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Colloquium
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Form of course completion
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Colloquium
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Accredited / Credits
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Yes,
1
Cred.
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Type of completion
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Combined
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Type of completion
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Combined
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Time requirements
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Lecture
1
[Hours/Week]
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Course credit prior to examination
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No
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Course credit prior to examination
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No
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Automatic acceptance of credit before examination
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No
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Included in study average
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NO
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Language of instruction
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Czech, English
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Occ/max
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Automatic acceptance of credit before examination
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No
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Summer semester
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0 / -
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0 / -
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0 / -
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Included in study average
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NO
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Winter semester
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0 / -
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0 / -
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0 / -
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Repeated registration
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NO
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Repeated registration
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NO
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Timetable
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Yes
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Semester taught
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Winter semester
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Semester taught
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Winter semester
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Minimum (B + C) students
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not determined
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Optional course |
Yes
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Optional course
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Yes
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Language of instruction
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Czech, English
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Internship duration
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0
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No. of hours of on-premise lessons |
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Evaluation scale |
S|N |
Periodicity |
každý rok
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Periodicita upřesnění |
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Fundamental theoretical course |
Yes
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Fundamental course |
No
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Fundamental theoretical course |
Yes
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Evaluation scale |
S|N |
Substituted course
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None
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Preclusive courses
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N/A
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Prerequisite courses
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N/A
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Informally recommended courses
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N/A
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Courses depending on this Course
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N/A
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Histogram of students' grades over the years:
Graphic PNG
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XLS
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Course objectives:
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To provide students with more detail knowledge of mass spectrometry.
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Requirements on student
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Student has to be able to discuss principles and influences of important parameters of a mass spectrometric measurement, to interpret mass spectra and to suggest suitable solutions of an analysis by mass spectrometry in different fields of application.
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Content
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Introduction to mass spectrometry, terminology, an origin of mass spectra; a scheme of mass spectrometer, resolution. Mechanisms of ionization, design of selected ion sources and their application (electron ionization - EI, chemical ionization - CI, atmospheric pressure chemical ionization - APCI, atmospheric pressure photoionization, electrospray ionization - ESI, desorption electrospray ionization - DESI, direct analysis in real time - DART, thermospray - TSP, fast atom (ion) bombardment - FAB, CF-FAB, FIB, field ionization - FI, field desorption - FD, laser desorption ionization - LDI, matrix assisted laser desorption ionization - MALDI, other desorption techniques). Design of mass analyzers - principles and theory of ion separation in a gas phase, fields of applications (sector instrument, quadrupole, ion trap, time of flight, ion cyclotron resonance, orbitrap). Tandem mass spectrometry - instrumentation (MS/MS, MSn). Induced dissociation (CID, SID). Detection and registration of ions, vacuum systems. Mass spectrometry in a structural analysis. Determination of molecular mass and elemental composition, a utilization of isotopic peaks, accurate mass. Nitrogen rule, double bond equivalent, identification of a structure type and functional groups. Fragmentation of ions after electron ionization, selected fragmentation processes, rearrangements. Metastable ions, overview of fragmentation of selected groups of organic compounds (hydrocarbons, halogen derivatives, alcohols and phenols, ethers, aldehydes and ketones, carboxylic acids, esters, nitrogen and sulfur compounds), procedure of a mass spectra interpretation. Quantitative analysis. Mass spectrometry of inorganic compounds.
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Activities
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Fields of study
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Guarantors and lecturers
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Literature
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Basic:
Dass Ch.: Fundamentals of contemporary mass spectrometry, John Wiley & Sons, Hoboken 2007..
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Basic:
de Hoffmann E., Stroobant, V.: Mass Spectrometry. Principles and Applications. John Wiley and sons, Chichester, 2002..
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Basic:
Gross J. H.: Mass Spectrometry, A Textbook, 3. vyd. Springer International Publishing AG, Cham 2017..
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Recommended:
Barker J.: Mass spectrometry, John Wiley and sons, Chichester, 1999..
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Recommended:
Cole, R. B. (editor): Electrospray ionization mass spectrometry, Wiley Chichester, 1997..
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Recommended:
Niessen W. M. A.: Liquid chromatography-mass spectrometry, 3. vyd. CRC Press, Taylor & Francis Group, Boca Raton 2006..
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Recommended:
Niessen W.M.A.: Liquid Chromatography-Mass Spectrometry, Marcel Dekker, New York, 1999..
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Recommended:
Scientific publications devoted to mass spectrometry (mass spectrometric and analytical journals)..
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Recommended:
Sparkman O. D., Penton Z. E., Kitson F. G.: Gas chromatography and mass spectrometry: a practical guide, 2. vyd. Academic Press, Burlington 2011.
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Recommended:
Technical and application notes of leading producers of mass spectrometers..
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On-line library catalogues
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Time requirements
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All forms of study
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Activities
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Time requirements for activity [h]
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Preparation for the Course Credit
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17
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Attendace
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13
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Total
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30
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Prerequisites - other information about course preconditions |
The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended. |
Competences acquired |
Define main concepts, describe main approaches, present the basic interpretation of mass spectra |
Teaching methods |
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Assessment methods |
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