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Course info
ACH / AHS
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Course description
Department/Unit / Abbreviation
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ACH
/
AHS
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Academic Year
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2024/2025
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Academic Year
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2024/2025
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Title
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Applied Mass Spectrometry
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Form of course completion
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Colloquium
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Form of course completion
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Colloquium
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Accredited / Credits
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Yes,
4
Cred.
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Type of completion
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Oral
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Type of completion
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Oral
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Time requirements
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Lecture
1
[Hours/Week]
Seminar
1
[Hours/Week]
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Course credit prior to examination
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No
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Course credit prior to examination
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No
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Automatic acceptance of credit before examination
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No
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Included in study average
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NO
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Language of instruction
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Czech, English
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Occ/max
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|
|
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Automatic acceptance of credit before examination
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No
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Summer semester
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0 / -
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0 / -
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0 / -
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Included in study average
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NO
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Winter semester
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0 / -
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0 / -
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0 / -
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Repeated registration
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NO
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Repeated registration
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NO
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Timetable
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Yes
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Semester taught
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Winter semester
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Semester taught
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Winter semester
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Minimum (B + C) students
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not determined
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Optional course |
Yes
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Optional course
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Yes
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Language of instruction
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Czech, English
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Internship duration
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0
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No. of hours of on-premise lessons |
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Evaluation scale |
S|N |
Periodicity |
každý rok
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Periodicita upřesnění |
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Fundamental theoretical course |
No
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Fundamental course |
No
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Fundamental theoretical course |
No
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Evaluation scale |
S|N |
Substituted course
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None
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Preclusive courses
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N/A
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Prerequisite courses
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N/A
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Informally recommended courses
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N/A
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Courses depending on this Course
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N/A
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Histogram of students' grades over the years:
Graphic PNG
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XLS
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Course objectives:
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To acquaint students with procedures and applications of mass spectrometry.
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Requirements on student
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Student has to prepare and give a talk concerning a selected topic, he has to be able to answer the related questions. During semester, he has to discuss the various topics with other students. During colloquium, student has to be able to give his opinion to any kicked around topic and to interpret mass spectra.
Student has to prepare and to give a talk concerning a selected topic, he has to be able to response related questions. During semester, he has to discuss with other students their topics. During colloquium, student has to be able to give his opinion to any kicked around topic and to interpret mass spectra.
|
Content
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Introduction, an application of mass spectrometry in organic analysis, history and development. Structural analysis of small organic molecules (structure verification, identification of impurities in pharmaceutical substances, identification of metabolites), determination of molecular mass and elemental composition, fragmentation after electron ionization, collision induced dissociation, fragmentation pathways and a study of fragmentation. Application of ESI-MS, an optimization of ion source, an analysis of small volumes of a sample using nano-ESI. MALDI-TOF in an analysis of polymers, practical problems of ionization. Hyphenation of mass spectrometry and separation techniques. GC/MS in a toxicological analysis (databases of spectra). HPLC/MS - an optimization of analytical methods, limits of used interfaces and their comparison (e.g. ESI vs. APCI). HPLC/MS in an environmental and pharmaceutical analysis. CE/MS - implementation of a method, selected applications. Mass spectrometry of peptides and their fragmentation (sequence of amino acid). Application of FTICR. Advances in mass spectrometry. Discussion of mentioned topics in a seminar and possible solution of corresponding analytical tasks (e.g. interpretation of spectra of small organic molecules, data evaluation).
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Activities
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Fields of study
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Guarantors and lecturers
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-
Guarantors:
prof. RNDr. Karel Lemr, Ph.D. (100%),
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Lecturer:
prof. RNDr. Karel Lemr, Ph.D. (100%),
Mgr. Volodymyr Pauk, Ph.D. (100%),
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Seminar lecturer:
prof. RNDr. Karel Lemr, Ph.D. (100%),
Mgr. Volodymyr Pauk, Ph.D. (100%),
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Literature
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Basic:
de Hoffmann E., Stroobant, V.: Mass Spectrometry. Principles and Applications. John Wiley and sons, Chichester, 2002..
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Basic:
Niessen W.M.A.: Liquid Chromatography-Mass Spectrometry, Marcel Dekker, New York, 1999..
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Recommended:
Cole, R. B. (editor): Electrospray ionization mass spectrometry, Wiley Chichester, 1997..
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Recommended:
Dass Ch.: Fundamentals of contemporary mass spectrometry, John Wiley & Sons, Hoboken 2007..
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Recommended:
Doporučená aktuální časopisecká literatura..
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Recommended:
Kinter M., Sherman N. E.: Protein Sequencing and identification using tandem mass spectrometry, John Wiley and sons, New York, 2000..
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Recommended:
Snyder A.P.: Interpreting protein mass spectra, American Chemical Society and Oxford University Press, Washington D.C., 2000..
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Recommended:
Technical and application notes of leading producers of mass spectrometers..
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Recommended:
Watson J.T.: Introduction to Mass spectrometry, Lippincott-Raven Publishers, Philadelphia, 1997..
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On-line library catalogues
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Time requirements
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All forms of study
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Activities
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Time requirements for activity [h]
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Attendace
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26
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Preparation for the Course Credit
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55
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Homework for Teaching
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39
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Total
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120
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Prerequisites - other information about course preconditions |
The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended. |
Competences acquired |
Apply theoretical knowledge for the solution of model problems in the field of mass spectrometry |
Teaching methods |
- Lecture
- Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Assessment methods |
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