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Course info
OCH / CNA
:
Course description
Department/Unit / Abbreviation
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OCH
/
CNA
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Academic Year
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2024/2025
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Academic Year
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2024/2025
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Title
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Chemistry of Nucleic Acids
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Form of course completion
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Exam
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Form of course completion
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Exam
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Accredited / Credits
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Yes,
3
Cred.
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Type of completion
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Combined
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Type of completion
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Combined
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Time requirements
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Lecture
2
[Hours/Week]
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Course credit prior to examination
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No
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Course credit prior to examination
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No
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Automatic acceptance of credit before examination
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No
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Included in study average
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YES
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Language of instruction
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English
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Occ/max
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Automatic acceptance of credit before examination
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No
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Summer semester
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0 / -
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0 / -
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0 / -
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Included in study average
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YES
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Winter semester
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0 / -
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0 / -
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0 / -
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Repeated registration
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NO
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Repeated registration
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NO
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Timetable
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Yes
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Semester taught
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Winter + Summer
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Semester taught
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Winter + Summer
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Minimum (B + C) students
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not determined
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Optional course |
No
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Optional course
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No
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Language of instruction
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English
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Internship duration
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0
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No. of hours of on-premise lessons |
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Evaluation scale |
A|B|C|D|E|F |
Periodicity |
každý rok
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Periodicita upřesnění |
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Fundamental theoretical course |
Yes
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Fundamental course |
No
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Fundamental theoretical course |
Yes
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Evaluation scale |
A|B|C|D|E|F |
Substituted course
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None
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Preclusive courses
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OCH/CNK
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Prerequisite courses
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N/A
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Informally recommended courses
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N/A
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Courses depending on this Course
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N/A
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Histogram of students' grades over the years:
Graphic PNG
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XLS
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Course objectives:
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The class is oriented on nucleosides, nucleotides and nucleic acids, their biological and chemical properties, synthesis and use.
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Requirements on student
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Successfully passing of a written exam (> 60 % of points) and success at an oral exam.
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Content
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Syllabus:
1. Introduction to the world of DNA and RNA. Historical overview of the main discoveries. Evolution.
2. Structure of DNA and RNA, nucleosides, nucleotides, physical and chemical properties. Primary and secondary structure of nucleic acids, occurrence and function in living organisms.
3. Nucleosides and nucleotides - chemical synthesis, chemistry of esters and anhydrides of phosphorus oxoacids.
4. Biosynthesis and metabolism of nucleosides, nucleotides and nucleic acids.
5. Synthesis of oligonucleotides in solution phase, on solid phase, biosynthesis of nucleic acids. Difference in approach in synthesis of DNA and RNA.
6. Modified nucleobases, modified backbone and modified sugar. Properties and preparation
7. Nucleic acids in biotechnology. PCR, DNA sequence determination, gene cloning, gene synthesis, detection of specific sequences by hybridization.
8. Genes and genome, gene structure, gene families, chromosomes, copying DNA (replication, transcription), mutations, genome repair, DNA recombination.
9. RNA structure and function, typical structural motifs, RNA processing and modification, translation, RNA involved in transport, RNA structure and function in viral systems.
10. Covalent interactions of nucleic acids and small molecules and repair of DNA lesions.
11. Protein-nucleic acids interactions. Features of nucleic acids recognized by proteins, representative recognition motifs, physical aspects of binding proteins to DNA, polymerases.
12. Physical and structural techniques applied to nucleic acids. Spectroscopic methods.
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Activities
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Fields of study
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Guarantors and lecturers
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Literature
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Recommended:
Blackburn, G.M. et al. Nucleic Acids in Chemistry and Biology.
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Recommended:
Blackburn, M. G., Gait, M. J., Loakes, D., Williams, D. M. Nucleic Acids in Chemistry and Biology. 3rd Ed.. Oxford: Oxford University Press, 2006.
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Recommended:
Lehninger. Principles of Biochemistry, W. H. Freeman and Co., NY, USA..
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On-line library catalogues
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Prerequisites - other information about course preconditions |
Interview in English language (general topic - should only reveal the communication skills in English). |
Competences acquired |
- |
Teaching methods |
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Assessment methods |
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