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Lecturer(s)
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Přichystalová Hana, Ing. Ph.D.
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Havlásek Jakub, Mgr.
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Hanyk Jiří, Mgr.
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Vostálová Jitka, doc. RNDr. Ph.D.
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Ulrichová Jitka, prof. RNDr. CSc.
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Franková Jana, doc. Mgr. Ph.D.
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Bancířová Martina, Mgr. Dr.
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Novotná Renata, Ing. Ph.D.
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Vrba Jiří, doc. Mgr. Ph.D.
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Zatloukalová Martina, Mgr. Ph.D.
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Zemanová Nina, Mgr.
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Rajnochová Svobodová Alena, doc. Ing. Ph.D.
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Ryšavá Alena, Mgr. Ph.D.
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Škařupová Denisa, Mgr.
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Šatka Štefan, Mgr.
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Kosina Pavel, Mgr. Ph.D.
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Dostál Zdeněk, Mgr. Ph.D.
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Dostálová Kateřina, Mgr. Ph.D.
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Kolářová Hana, prof. RNDr. CSc.
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Course content
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Biochemistry, introduction. Proteins, structure, function. Supramolecular assemblies. Heteroproteins. Structure and function of hemoglobin. Immunoglobulins. Enzymes-structure, classification, mechanism of action. Cofactors and vitamins. Enzyme activity and its regulation. Enzymes in diagnosis. Signal transduction. Mechanism of hormone action. Biochemical function of subcellular organelles and membrane systems. Introduction to metabolic pathways. Bioenergetics. High-energy compounds. Biological oxidation. Respiratory chain, oxidative phosphorylation. Metabolism-overwiev. Citric acid cycle. AcetylCoA-central role in metabolism. Carbohydrate metabolism, overview. Glycolysis. Gluconeogenesis, pentose phosphate pathway, glycogen metabolism. Biomacromolecules in medicine. Rules of study and directions for laboratory work. Basic laboratory techniques. Selected instrumental techniques, chromatography, electrophoresis, spectrophotometry. Preparation of solutions, determination of concentration, measurement of pH. Measurement and calculations of pH and buffers capacity. Structure of biochemically important compounds. Properties of amino acids and proteins. Determination of isoelectric points of histidine. Isolation of albumins and globulins. Electrophoretic separation of proteins and acidic, neutral and basic amino acids. Stanovení proteinů. Properties and chemical reactions saccharides. Properties and chemical reactions of lipids. Identification of levomepromazine and its metabolites in biological materials. Preparation of deionizated solvents by ion exchanged chromatography. Gel chromatography of hemolyzed blood. Quantitative determination of salicylates, vitamin C a acytetylsalicylic acid.
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Learning activities and teaching methods
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Lecture, Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook), Demonstration, Training in job and motor Skils
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Learning outcomes
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The aim of the subject is to explain principles of biochemistry and the regulatory mechanisms of basic metabolic pathways. This knowledge is essential for interpretation of physiological and pathological events in human organism. Students are also trained in laboratory classes with res-pect to master simple biochemical analyses and the ability to interpret the results from laboratory tests.
The student who passes the subject will be equipped with knowledge on basic biochemical pathways and information on regulation of intermediary metabolism. Student will be able to interpret physiological and pathological events in human organism. In practice, student will be able to work according to good laboratory practice rules and to perform basic biochemical tests and to interpret laboratory results.
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Prerequisites
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Passing subject Medical chemistry.
LCH/VCA11 and BIO/VCA11
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Assessment methods and criteria
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Mark, Written exam
100% attendance in practicals
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Recommended literature
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Devlin, T. M. (2010). Textbook of Biochemistry with Clinical Correlations. John Wiley & Sons.
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Dobrota, D. (2012). Lekárska biochémia. Martin: Osveta.
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Dvořáčková, S. a kol. (2007). Praktikum lékařské chemie a biochemie. Olomouc - UP.
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Champe, P. C., Harvey, R. A., Ferrier, D. R. (2007). Biochemistry. Lippincott Williams & Wilkins.
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Koolman, J., Röhm, K. H. (2012). Barevný atlas biochemie. Praha: Grada.
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Ledvina, M. a kol. (2009). Biochemie pro studující medicíny (1. a 2. díl). Praha - Karolinum, UK.
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Matouš, B. a kol. (2010). Základy lékařské chemie a biochemie. Praha: Galén.
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Murray, R. K. a kol. (2002). Harperova biochemie. Jinočany: H&H.
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Murray, R. K. a kol. (2012). Harperova ilustrovaná biochemie. Praha: Galén.
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Newsholme, E., Leech, A. (2010). Functional Biochemistry in Health and Disease. Wiley.
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Racek, J. a kol. (2006). Klinická biochemie. Praha: Galén.
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