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Lecturer(s)
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Tarkowská Danuše, Mgr. Ph.D.
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Course content
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Introduction, survey of techniques, terminology. Sample handling. Development of purification method. Trace analysis requirements. Mechanical processing in sample preparation. Membrane separation. Headspace sampling techniques. Phase transfer separations. Sonication as sample preparation method. Microwave based extraction. Extraction techniques - solvent extraction, sorbent extraction. Preparative chromatography. Immunoaffinity purification methods. Electroseparation techniques in sample preparation. Sample derivatization. Automation in sample preparation.
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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Introduction, survey of techniques, terminology.
Student should be able to (after attending the course): - apply basic methods for preparation of a biological material
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Prerequisites
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unspecified
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Assessment methods and criteria
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Mark
80% fruitfulness in imposition
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Recommended literature
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Churáček J. a kol. (1990). Analytická separace látek, 1. vyd.. Státní nakladatelství technické literatury, Praha.
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Churáček J. a kol. (1993). Nové trendy v teorii a instrumentaci vybraných analytických metod. Praha.
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