Lecturer(s)
|
-
Oklešťková Jana, Mgr. Ph.D.
|
Course content
|
Immune system, immune response, immunoglobulins, molecular biologie of antibodies, immunochemicals methods, monoclonal, polyclonal and recombinant antibodies, antigen, haptens, methods of conjugation, immunozation, isolation and purification of antibodies; Monoclonal antibodies: hybridoma technologies, characteristic of splenocytes, myeloma cells and hybridomas, growing hybridomas, in vitro and in vivo production methods, immunoassays, biotechnologies, advantages and disadvantages of monoclonal, polyclonal and recombinat antibodies.
|
Learning activities and teaching methods
|
Lecture
- Homework for Teaching
- 15 hours per semester
|
Learning outcomes
|
To lern introduced section of hybridoma and recombinant technologies.
Student should be able to (after attending the course): - characterise the immune response; - recall the principles of immunochemicals methods; - explain the differences between monoclonal, polyclonal and recombinant antibodies; - describe and interpret hybridoma technologies; - judge the advantages and disadvantages of monoclonal, polyclonal and recombinat antibodies;
|
Prerequisites
|
Students should have a basic knowledge of immunology.
|
Assessment methods and criteria
|
Written exam
75% correctly answered questions in written test
|
Recommended literature
|
-
auct. (2007). Fundamental Techniques in Cell Culture: A Laboratory Handbook. Sigma-Aldrich.
-
Ferenčík M. (1989). Imunochémia. Alfa, Bratislava.
-
Harlow E., Lane D. (1988). Antibodies a laboratory manual. Cold Spring harbor Lab. USA.
-
McCullough K., Spier R. E. (1990). Monoclonal antibodies in biotechnology: theoretical and practical aspects. Cambridge University Press, Cambridge, UK.
-
Voet D., Voetová J. G. (1990). Biochemie. Victoria Publishing, Praha.
|