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Lecturer(s)
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Fellner Martin, prof. RNDr. Ph.D.
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Ohnoutková Ludmila, Ing. Ph.D.
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Vlčko Tomáš, Mgr.
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Course content
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Laboratory practice will be focused on the methods of molecular and cell biology and their applications. The laboratory course will focus on molecular biology methods and their applications. 1) Vector linearization, electrophoresis, gel extraction using columns, 4 hours (odd-numbered week) 2) Ligation of insert into vector, bacterial transformation, cultivation, 4 hours (odd-numbered week) 3) Colony PCR, electrophoresis, cultivation, 4 hours (odd-numbered week) 4) Vector linearization, Gateway cloning, 4 hours (odd-numbered week) 5) Transformation of explants with *A. tumefaciens*, preparation of culture medium, 4 hours (odd-numbered week) 6) qPCR, reaction process, quantification, 4 hours (odd-numbered week) 7) Colloquium, 2 hours.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration
- Attendace
- 24 hours per semester
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Learning outcomes
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Molecular biology is the scientific discipline that deals with the study of biological processes and their mutual functional relations at the molecular level. The principles and techniques are applied in various disciplines, such as genetic engineering.
After passing the course, the student should be able to: - Describe principles of molecular techniques and ways of their applications. - Name basic procedures and methods, including applications. - Characterize genetically modified organisms. - Prepare plant tissues and cells for genetic manipulations.
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Prerequisites
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Student should have basic knowledge of molecular biology
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Assessment methods and criteria
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Student performance
Attendance at exercises 100%; mastering the issue.
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Recommended literature
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Brown T.A. (2007). Klonování genů a analýza DNA. V Olomouci 2007.
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Buchanan B.B., Gruissem W., Jones R.L. (2001). Biochemistry and Molecular Biology of Plants.. Soc. Plant Physiol. Rockville.
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Jackson F., Linskens H.F. (2000). Genetic Transformation of Plants (Molecular Methods of Plant Analysis).. Springer.
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Ondřej M., Drobník J. (2002). Transgenoze rostlin.. Akademia.
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Reece J. (2004). Analysis of Genes and Genomes.. West Sussex.
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