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Lecturer(s)
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Brabec Viktor, prof. RNDr. DrSc.
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Course content
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DNA conformation. A-, B-, Z- forms. Twist, tilt. DNA curvature. Helix-coil transition, forces stabilizing double helix. Higher organization of DNA, supercoiled DNA. Condensed forms. Chromosomes, nucleosomes, chromatin. Interactions of DNA with proteins. Interaction of DNA with ions. Protonation, pK, metal ions, electrostatic shielding. Structure and properties of RNA. Double-helical RNA. A-, Z- forms of RNA. Higher organization of RNA, t-RNA, ribosomes. Hydrodynamic and spectral properties of nucleic acids. Viscosity, sedimentation, diffusion. Osmosis. Electron vibration absorption spectra, hypochromicity, melting temperature. Spectroscopy of polarized light, ORD and CD. Genetic information. Replication, transcription, translation. Genetic code. Codon. RNA-polymerases. Viruses. Retroviruses. Reverse transcription. Mutations. Recombination. Oncogens.
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Learning activities and teaching methods
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Lecture, Work with Text (with Book, Textbook)
- Preparation for the Exam
- 70 hours per semester
- Homework for Teaching
- 30 hours per semester
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Learning outcomes
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DNA conformations. Structure and properties of RNA. Hydrodynamic and spectral properties of nucleic acids.
Evaluation Evaluate the particular methods and principles, explain the aspects and results concerning the given issue, integrate the knowledge, predict the solutions, evaluate the results and outcomes.
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Prerequisites
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Basic knowledge of biochemistry, molecular biophysics and molecular biology.
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Assessment methods and criteria
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Oral exam
Knowledge within the scope of the course topics (examination)
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Recommended literature
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Lenaz G., Milazzo D. (ed.). (1997). Bioelectrochemistry of Biomacromolecules. Birkhauser Verlag, Basel, Boston, Berlin.
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Rosypal S. (2000). Úvod do molekulární biologie, I, II, III. Brno.
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