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Lecturer(s)
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Jedelská Tereza, Mgr. Ph.D.
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Luhová Lenka, doc. RNDr. Ph.D.
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Course content
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1. Basic characteristics of enzymes, cofactors. Nomenclature of enzymes. Localization of enzymes. Structure of enzymes 2. Isolation, purification procedures. Methods of studying the active centre. Covalent modification of enzymes 3. Mechanism of enzyme catalysis. Michaelis-Menten equations, Michaelis constant, reaction order, enzyme units, conversion number. 4. Effect of environment on enzyme activity. 5. One, two and three-substrate reactions. 6. Enzyme specificity, allosteric regulation, covalent modification. 7. Enzyme inhibition. 8. Methods for the determination of enzyme activity. 9. Enzyme analytical techniques 10. Use of enzymes in practice. A 11. Clinical aspects of enzymology. 12. Enzymes for molecular biology.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 13 hours per semester
- Preparation for the Exam
- 10 hours per semester
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Learning outcomes
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Introduction to the question of enzymes: their basic characterization, kinetics of reactions, determination of activity and use in practice.
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Prerequisites
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Basic knowledge of biochemistry.
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Assessment methods and criteria
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Written exam
Written exam of 1 hour, 12 questions, maximum score 30. A 30-28 (100-93%) B 27-25 (90-83%) C 24-22 (80-73%) D 21-19 (70-63%) E 18-15 ( 59-50%) F: < 15 (< 50%)
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Recommended literature
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Bhatt, S. K., Khan, A. M, Thapa, N. (2020). Text Book Of Enzymology: Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subjects.. LAP LAMBERT Academic Publishing.
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Bisswanger H. (2011). Practical Enzymology. Weinheim, Wiley.
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Bisswanger, H. (2019). Practical Enzymology. Wiley-VCH Verlag GmbH.
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Blume, P, Freier, E. F. (2015). Enzymology in the Practice of Laboratory Medicine. Academic Press.
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Bugg T. D. H. (2012). Intoduction to enzyme and coenzyme chemistry 3rd Ed.. WestSussex UK.
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Cornish-Bowden A. (2012). Fundamentals of Enzyme Kinetics,Weinheim, Germany,. Weinheim, Germany.
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Macholán, L., Barthová, J., Kučera, I. (1994). Enzymologie. MU Brno.
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Marangoni A. G. (2003). Enzyme Kinetics a Modern Approach. New Jersey.
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Price N. C., Stevens L. (2000). Fundamentals of enzymology. New York, USA.
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Purich D. L. (2010). Enzyme Kinetics: Catalysis and Control. London.
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