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Lecturer(s)
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Danihlík Jiří, Mgr. Ph.D.
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Sekaninová Jana, Mgr. Ph.D.
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Zalabák David, Mgr. Ph.D.
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Janků Martina, Mgr. Ph.D.
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Dostálková Silvie, Mgr. Ph.D.
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Vrobel Ondřej, Mgr. Ph.D.
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Hrtánková Eva, Mgr.
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Jemelková Jana, Mgr. Ph.D.
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Jedelská Tereza, Mgr. Ph.D.
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Course content
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Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) followed by western blotting. Immunochemical detection of proteins transferred on PVDF membrane. Analysis of sugars using HPLC. Analysis of biogenic amines using liquid chromatography coupled with mass spectrometry. Light microscopy. Isoelectric focusing. Bioinformatic approach to protein analysis. PCR - identification of transformed bacterial colonies. Enzyme kinetics.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
- Attendace
- 52 hours per semester
- Homework for Teaching
- 20 hours per semester
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Learning outcomes
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The aim of the course is to teach students to implement in practice basic separation and instrumental methods used in biochemical laboratories.
Understanding the principles of methods and gaining practical experience in the implementation of basic and advanced biochemical methods.
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Prerequisites
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The knowledge and skills acquired by taking courses in biochemistry and biochemical methods, as well as laboratory exercises in biochemistry and laboratory techniques, are required.
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Assessment methods and criteria
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Student performance, Systematic Observation of Student, Written exam
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Recommended literature
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Katoch, R. (2011). Analytical techniques in biochemistry and molecular biology. Springer, New York.
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Králová, B. (2001). Bioanalytické metody. VŠChT.
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Nováková, L., Douša, M. a kol. (2013). Moderní HPLC separce v teorii a praxi I. Europrint, 2013.
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Nováková, L., Douša, M. a kol. (2013). Moderní HPLC separce v teorii a praxi II. Europrint, 2013.
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Pingoud, A., Urbanke, C., Hoggett J., Jeltsch, A. (2002). Biochemical Methods, A Concise Guide for Students and Researchers. Wiley-VCH, Weinheim.
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Šebela, M. a kol. (2016). Experimentální metody biochemie (elektronická skripta). Olomouc.
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Vashist S., Luong J. (2018). Handbook of Immunoassay Technologies 1st Edition. Academic Press.
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