Course: Advanced Biochemistry

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Course title Advanced Biochemistry
Course code KBC/PBC
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Vostálová Jitka, doc. RNDr. Ph.D.
Course content
1. Immunity. Structure and function of immunoglobulins, immune response, vaccination. 2. Oxygen transport. Structure and function of haemoglobin and myoglobin. Allostery. Role of iron. 3. Digestive system. Process of digestion of nutrients (carbohydrates, lipids and proteins), digestive enzymes. 4. The liver. Central role in nutrient metabolism. Metabolism of carbohydrates, proteins, lipids, nitrogen and nitrogenous substances, metabolism of bilirubin, formation of ketone bodies, metabolism of xenobiotics and alcohol. 5. Lipoproteins and lipid transport. Apoproteins, lipoprotein receptors and enzymes involved in lipoprotein metabolism. 6. Cholesterol and steroid substances. Synthesis and function of cholesterol, bile acids, steroid hormones and vitamin D3. 7. Complex lipids. Glycerophospholipids, sphingolipids, cerebrosides, gangliosides. 8. Glycoproteins and proteoglycans. Structure, biosynthesis, function. 9. Homeostasis of nutrient metabolism. Nutrition, regulation of energy metabolism. Function of insulin and glucagon. Pathobiochemistry of carbohydrates (Diabetes mellitus, disorders of carbohydrate metabolism). 10. Biochemistry of connective tissue. Extracellular matrix proteins - collagen, elastin and non-collagenous proteins. Biochemistry of bone. 11. Muscle. Muscle structure, biochemistry of smooth and skeletal muscle contraction. 12. Minerals. Role of potassium, sodium, magnesium, calcium and trace elements. 13. Endocrinology. Classification of hormones, mechanism of action, steroid and peptide hormones, catecholamines, thyroid hormones. 14. Neurochemistry. Neurotransmitters, biochemistry of nerve transmission and vision, neurodegenerative diseases.

Learning activities and teaching methods
Lecture
  • Attendace - 26 hours per semester
  • Preparation for the Exam - 60 hours per semester
Learning outcomes
Deepen the knowledge of biochemistry acquired as part of the bachelor's study. Finding the connection between metabolic processes and physiological ones manifestations of living objects, e.g.: biochemistry of vision, digestion, nervous, activities, with an emphasis on signaling and regulatory mechanisms.
Finding the link between metabolic processes and physiological manifestations of living objects, e.g.: biochemistry of vision, digestion, nervous, activities, with emphasis on signaling and regulatory mechanisms.
Prerequisites
Knowledge of basic biochemistry, molecular biology, animal physiology or pathophysiology.

Assessment methods and criteria
Mark, Oral exam

Oral exam
Recommended literature
  • Devlin, T. M. (2010). Textbook of Biochemistry with Clinical Correlations. John Wiley & Sons.
  • Dobrota, D. (2012). Lekárska biochémia. Martin: Osveta.
  • Ferrier, D.S. (2018). Biochemistry. Lippincott Illustrated Reviews. Walters Kluwer.
  • Champe, P. C., Harvey, R. A. (2014). Biochemistry (6th Edition). Lippincott Williams & Wilkins.
  • Matouš, B. (2010). Základy lékařské chemie a biochemie. Galén.
  • Murray, R. K. a kol. (2012). Harperova ilustrovaná biochemie. Praha: Galén.
  • Murray R. K., Bender D. A., Botham K. M., Kennelly P. J., Rodwell V. W., Weil P. A. Harpers. (2012). Illustrated Biochemistry (29th ed.). Lange Medical Book.
  • Vasudevan, D.M., Spreekumari, S., Vaidyanathan, K. (2015). Úvod do všeobecnej a klinicky aplikovanej biochémie. Bratislava: Balneotherma s.r.o.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biochemistry (2022) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Analytical Biochemist (2024) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Summer