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Lecturer(s)
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Raška Milan, prof. Mgr. MUDr. Ph.D.
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Kosztyu Petr, Mgr. Ph.D.
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Zachová Kateřina, Mgr. Ph.D.
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Course content
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The student will learn about collection of biological material, transport of biological material, storage of material and suitability for individual laboratory determinations. - Agglutination reactions - Complement fixation reactions - Immunoprecipitation reactions - Simple radial immunodiffusion - RID - Double (reverse) immunodiffusion - Precipitation in liquid medium (nephelometry, turbidimetry) - Indirect immunofluorescence - ELISA - Determination of antibody affinity by SPR - Evaluation of neutralizing antibodies - Immunoblotting methods - Determination of antiplatelet antibodies - Determination of allergen-specific IgE and autoantibodies by FEIA-CAP - Examination of phagocytosis by Candida albicans - Granulocyte chemotaxis testing by migration under agarose - Burst test - respiratory burst (dihydrorhodamine R123) - Flow cytometry - determination of leukocyte subpopulations - Flow cytometry - determination of antigen-specific T lymphocyte subpopulations - ELISPOT methods - determination of IFN production by stimulated lymphocytes - Examination of the HLA system - HLA typing by serological technique - HLA typing by molecular genetic (DNA) methods - Examination of anti-HLA antibodies - Evaluation of TREC and KREC quantification - Investigation of mutations and polymorphisms by sequencing - Sanger sequencing principle - Next Generation Sequencing (Next Generation Sequencing) and applications in immunology
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Learning activities and teaching methods
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Lecture
- Attendace
- 13 hours per semester
- Preparation for the Exam
- 20 hours per semester
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Learning outcomes
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The course is to introduce students to modern immunological methods used in diagnostics. The aim is to give an overview of the methods routinely performed in clinical laboratories, as well as the most modern methods and to introduce their principles, and partly also their indications, reference values and clinical interpretation.
The student will gain knowledge of basic and advanced diagnostic methods used in immunology.
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Prerequisites
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Basic knowledge of biochemistry, clinical biochemistry, animal physiology. At the same time it is necessary to have enrolled in the course KBC/ZIMU Fundamentals of Immunology.
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Assessment methods and criteria
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Written exam
The written examination in the form of test questions confirming knowledge of the taught material is 1 hour long, 25 questions, maximum score 25. A: 25-23 points B: 22-20 points C: 19-17 points D: 16-14 points E: 13-12 points F: less than 12 points
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Recommended literature
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Abbas, A. K., Lichtman, A. H., Pillai, S. (2007). Cellular and Molecular Immunology. Saunders.
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Bartůňková, J., Paulík, M., a kol. (2011). Vyšetřovací metody v imunologii. Praha: Grada.
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Janeway, Ch. A., Travers, P., Walport, M. (2018). Immunobiology. Garland Science New York.
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Krejsek, J., Kopecký, O. (2004). Klinická imunologie. Nucleus HK.
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Křupka, M., Raška, M., et al. (2020). Imunologické vyšetřovací metody. LF UP Olomouc.
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