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for academic year 2025/2026
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Course: Practice of molecular-biological methods in diagnostics

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Course title Practice of molecular-biological methods in diagnostics
Course code KBC/MBM
Organizational form of instruction Exercise
Level of course Master
Year of study 1
Semester Summer
Number of ECTS credits 7
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Zalabák David, Mgr. Ph.D.
  • Škrabišová Mária, Mgr. Ph.D.
Course content
1. Identification of bacterial plasmid by restriction analysis (plasmid isolation, restriction reaction, agarose gel electrophoresis). 2. Detection of SARS-CoV-2 viral disease by quantitative PCR 3. Detection of mutant allele of human Factor V Leiden gene by quantitative PCR 4. PCR genotyping of T-DNA insertional mutants of the model plant Arabidopsis thaliana 5. Detection of bee pathogens by loop-mediated isothermal amplification (LAMP)

Learning activities and teaching methods
Laboratory Work, Group work
  • Attendace - 104 hours per semester
  • Homework for Teaching - 10 hours per semester
Learning outcomes
This is a block exercise, which is thematically related to the course Molecular Biological Diagnostic Methods (KBC/MBDM). In this exercise, students will practically try modern molecular biological diagnostic methods. The exercise is designed to cover the scope of diagnostics in medicine, plant biology and veterinary diagnostics.
The student will gain practical experience with the implementation of molecular biological methods used in diagnostics.
Prerequisites
Absolvování předmětu KBC/MBDM.
KBC/MB and KBC/MBDM

Assessment methods and criteria
Student performance, Systematic Observation of Student

The student must complete all experimental tasks, achieve 80% of the possible points (the quality and accuracy of the work is evaluated according to the results) and prepare protocols on the solution of the tasks. The KBC/MB and KBC/MBDM exams must be passed before entering the exercise.
Recommended literature
  • Cooper, P. C., Rezende, S. M. (2007). An overview of methods for detection of factor V Leiden and the prothrombin G20210A mutations. Int. Jnl. Lab. Hem., 29, 153?162.
  • Liang, F. T., Steere, A. C., Marques, A. R., Johnson, B. J., Miller, J. N., Philipp, M. T. Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi vlsE. J Clin Microbiol. 1999 Dec;37(12):3990-6.
  • Magnarelli, L. A., Anderson, J. F., Barbour, A. G. Enzyme-Linked Immunosorbent Assays for Lyme Disease: Reactivity of Subunits of Borrelia burgdorferi. The Journal of Infectious Diseases, Volume 159, Issue 1, January 1989, Pages 43?49.
  • Malekshahi, A., Khanizadeh, S., Fallahi, S. et al. (2022). Diagnostic power of one-step and two-step RT-qPCR methods to SARS?CoV?2 detection. BMC Infect Dis 22, 505.
  • Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., Hase, T. (2000). Loop-mediated isotherma amplification of DNA. Nucleic Acids Research, 28, 12, e63.
  • O'Malley, R. C., Barragan, C. C., Ecker, J. R. A user's guide to the Arabidopsis T-DNA insertion mutant collections. Methods Mol Biol. 2015;1284:323-42.
  • Różański, M., Walczak-Drzewiecka, A., Witaszewska, J. et al. (2022). RT-qPCR-based tests for SARS-CoV-2 detection in pooled saliva samples for massive population screening to monitor epidemics. Sci Rep 12, 8082.
  • Stepanova, A. N., Alonso, J. M. PCR-based screening for insertional mutants. Methods Mol Biol. 2006;323:163-72.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Analytical Biochemist (2024) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Summer
Palacký University Olomouc, date of update: 25.10.2025 23:57. Data created for academic year 2025/2026