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Lecturer(s)
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Rašková Michaela, Mgr.
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Urva Pavel, Mgr.
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Slivková Jana, Mgr.
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Miková Eva, Mgr. Ph.D.
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Janků Martina, Mgr. Ph.D.
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Jedelská Tereza, Mgr. Ph.D.
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Jemelková Jana, Mgr. Ph.D.
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Jahnová Jana, Mgr.
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Myslínová Kristýna, Mgr.
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Dostálková Silvie, Mgr. Ph.D.
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Course content
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Basic skills in biochemistry laboratory: weights, pipetting technique, dilution series, preparation of buffers. Basic methods in biochemistry laboratory: homogenization of biological material, extraction and precipitation, filtration and centrifugation, membrane separation (dialysis and ultrafiltration), spectrophotometry (calibration curve, concentration measurements), chromatography (gel permeation chromatography, TLC), electromigration methods (agarose electrophoresis), distillations, sublimation, melting point determination, titration, conductometry, microscopic methods and analyses.
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Learning activities and teaching methods
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Demonstration, Laboratory Work
- Homework for Teaching
- 50 hours per semester
- Attendace
- 65 hours per semester
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Learning outcomes
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Basic laboratory procedures and methods used in a biochemical laboratory.
Apply and practice fundamental laboratory operations.
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Prerequisites
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Student of the Biochemistry or BGI study program.
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Assessment methods and criteria
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Written exam, Student performance
1. 100% attendance at the exercise (possibility of replacing up to 2 absences for serious reasons, exceptional situations will be dealt with individually, e.g. hospital stay). Any absence must always be excused in writing (e-mail) in advance before the start of the exercise. 2. Submitted and recognized protocols from all exercises. A pair of students solving a given task together will submit one protocol in electronic form (as a text document). The prepared protocols must be submitted no later than the beginning of the following exercise (or within 7 days in the case of replacing an exercise and also in the case of the last exercise). For each protocol submitted on time and correctly prepared, the student will receive 10 points. In the event of failure to meet the deadline for submitting the protocol, 5 points will be deducted. The protocol is corrected and scored after submission. In the case of correction of the protocol, each corrected version of the protocol must be submitted within a week of its receipt. For each repeatedly returned correction of the protocol, 2 points will be deducted. The protocol must always be recognized by the exercise leader. The exception is the protocol from the last completed exercise. This protocol is not returned for correction, only points are deducted appropriately for errors and shortcomings. All protocols must be recognized no later than Friday 9th January 2026. 3. During the exercise, the student's readiness will be tested in the writting (5 questions from the theoretical and practical parts of the given task, 1 question is a computational example). Each correctly answered question is awarded 1 point. If the student receives less than 2 points from the test, the student is expelled from the exercise and must repeat the exercise on an alternate date. If the student receives 2-3 points, the supervisor is obliged to test the student orally (2 questions from theoretical part and one question from practical part), whereby the student must answer at least 2 correctly; in case of ignorance of the given topic, the student is expelled from the exercise and must repeat the exercise on an alternate date. Due to unpreparedness, it is possible to replace the exercise only once. 4. The student must meet all requirements for completing the course and obtain the required number of points for readiness and submitted protocols, within two weeks of the last exercise. Two weeks after the end of the exercise, the point gain and fulfillment of the requirements for obtaining credit for this course are evaluated, i.e. 100% participation in the exercise and all protocols submitted and recognized. Points evaluation criteria: 75 - 100% points - Credit is awarded (1st regular attempt). 50 - 75% points - Student will write a credit test (the test must be written with more than 70% points to be awarded credit, the credit test can be repeated only once (2nd and 3rd regular attempt). < 50% points - Student will not be awarded credit. The student must repeat the course the following academic year.
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Recommended literature
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Anzenbacher P., Kovář J. (1986). Metody chemického výzkumu pro biochemiky. MŠ ČSR Praha.
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Galuszka P., Luhová L. Laboratorní technika pro biochemiky, UP Olomouc, 2003.
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Peč P. a kol. Laboratorní cvičení z biochemie, UP Olomouc, 2000.
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Pingoud A., Urbanke C., Hoggett J., Jeltsch A. (2002). Biochemical Methods: A Concise Guide for Students and Researchers.. Wiley-VCH.
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