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Lecturer(s)
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Friedecký David, prof. RNDr. Ph.D.
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Kvasnička Aleš, Mgr. Ph.D.
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Kouřil Štěpán, Mgr. Ph.D.
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Dobešová Dana, Mgr.
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Course content
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Metabolomics and lipidomics are representatives of the so-called omics methods, where a combination of separation techniques with mass spectrometry is applied to study the complex profile of metabolites (metabolome) and lipids (lipidome). These methods have found widespread application both in the understanding of pathobiochemical processes and in the search for new biomarkers in the diseases under study. The exercise will build on the theoretical knowledge acquired in the KBC/OM course Omics Methods in Diagnostics and will consist of performing the entire metabolomic and lipidomic workflow on selected biological samples. Students will be introduced in detail to study design, sample preparation, performing the actual sample analysis, processing of measured mass spectrometry records, data transformation, statistical evaluation and application of bioinformatics tools. Finally, students will conduct a research on a given topic to evaluate the contribution of omics methods in relation to the known information about the disease.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
- Attendace
- 52 hours per semester
- Homework for Teaching
- 15 hours per semester
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Learning outcomes
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The aim of the course is to provide students with a practical introduction to the methods used in metabolomics and lipidomics.
Students will learn how to proceed in metabolic studies by preparing samples, performing the actual analysis of samples, processing measured mass spectrometry records, transforming data, statistical evaluation, and applying bioinformatics tools.
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Prerequisites
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The student knows the basic principles of analytical methods such as chromatographic methods and mass spectrometry.
KBC/OM
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Assessment methods and criteria
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Student performance, Written exam
Written tests, elaborated protocols.
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Recommended literature
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Edited ByRon Wehrens, Reza Salek. Metabolomics, Practical Guide to Design and Analysis, 2019, eBook ISBN9781315370583.
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Editors: Alessandra Sussulini. Metabolomics: From Fundamentals to Clinical Applications, 2017, ISBN: 978-3-319-47656-8.
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Editors: Martin Giera. Clinical Metabolomics, Methods and Protocols, 2018, ISBN: 978-1-4939-7592-1.
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Editors: Paul Wood. Lipidomics, 2017, ISBN: 978-1-4939-6946-3.
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