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Lecturer(s)
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Danihlík Jiří, Mgr. Ph.D.
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Sekaninová Jana, Mgr. Ph.D.
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Course content
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Basics of Clinical Laboratory Work and Blood Collection at the Olomouc University Hospital Lipids (Spectrophotometric determination of triacylglycerols and cholesterol in blood serum) Glucose (Spectrophotometric determination of glucose in blood serum, glycemic curve) Urine parameters (Determination of basic urine parameters by test tube reactions and diagnostic strips) Blood serum proteins (Spectrophotometric determination of albumin and total proteins, blood serum electrophoresis) Immunomethods (Determination of antibodies against EBV (or Borrelia) using a commercial ELISA kit, agglutination test for the determination of CRP and Antistreptolysin) Determination of nitrogenous non-protein substances (Spectrophotometric determination of urea, uric acid and creatinine) Determination of Inorganic Ions (Spectrophotometric Determination of Ca, Mg, P) Determination of iron and blood group (Spectrophotometric determination of iron in blood serum, determination of blood group by agglutination test)Determination of enzyme activity (Spectrophotometric determination of lactate dehydrogenase or amylase activity) Excursion to the laboratories of clinical biochemistry of the Olomouc University Hospital or to the Transfusion Department of the Olomouc University Hospital
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
- Attendace
- 52 hours per semester
- Homework for Teaching
- 30 hours per semester
- Preparation for the Course Credit
- 30 hours per semester
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Learning outcomes
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Main approaches used in clinical biochemistry. Practical application of methods in clinical laboratories.
Orientation (understanding) and independent work with commercial kits for the analysis of biochemical parameters in blood serum.
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Prerequisites
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Basic kowledge from biochemistry
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Assessment methods and criteria
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Student performance, Systematic Observation of Student
1) 100% participation in the exercise (possibility of replacing up to 2 absences for serious reasons, exceptional situations will be dealt with individually, e.g. hospital stay). Any absence must always be excused in writing (email) in advance before the start of the exercise. 2) Submitted and recognized protocols from all exercises. Each student submits their own protocol in electronic form. The prepared protocols must be submitted no later than the beginning of the following exercise (or within 7 days in the case of replacing the exercise and the last exercise)! For each protocol submitted on time and correctly prepared, the student receives 1 point. In case of failure to meet the deadline for submitting the protocol, 1 point is deducted from the student. The protocol is corrected and scored after submission. The protocols are no longer subject to further corrections. 3) During the exercise, the student's readiness will be tested in the form of a test (5 questions from the theoretical and practical parts of the given task). Each correctly answered question is valued at 2 points. If the student receives 3 or fewer points from the test, the student is expelled from the exercise and must repeat the exercise on an alternate date. If the student receives 4-5 points, the supervisor is obliged to test the student orally; in case of ignorance of the given topic, the student is expelled from the exercise and must repeat the exercise on an alternate date. Due to unpreparedness, it is possible to replace the exercise only once. 4) The student must meet all requirements for completing the course and obtain the necessary number of points for readiness and submitted protocols, within two weeks of the last exercise. Two weeks after the end of the exercise, the point gain and the fulfillment of the requirements for obtaining credit for this course are evaluated, i.e. 100% participation in the exercise and all protocols submitted and recognized. Point evaluation criteria: 75 - 100% points - Credit is awarded (1st regular attempt). 50 - 75% points - The student will write a credit test (the test must be written with more than 70% points to be awarded credit, the credit test can be repeated only once (2nd and 3rd regular attempts). < 50% points - The student will not be awarded credit, he must register for the course again next year.
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Recommended literature
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Brodská HL, Kohout P a kol. Laboratorní vyšetření v klinické praxi. 2022.
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Kaplan and Pesce. Clinical chemistry: theory, analysis, correlation. USA: Mosby Elsevier. 2010.
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Racek J. a kol. Klinická biochemie. Praha: Galén. 2021.
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Zajoncová, L. (2013). Praktická cvičení z klinické biochemie. UP Olomouc.
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Zima T. a kol. Laboratorní diagnostika. Praha: Galén. 2017.
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