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Lecturer(s)
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Tománková Kateřina, RNDr. Ph.D.
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Course content
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1. Department of Plant Pest Diagnostics - presentation of laboratory activities. Accreditation of laboratories according to EN ISO/IEC 17025:2018. Tour of laboratories. 2. Diagnostic methods used in the Laboratory of Bacteriology of the Institute of Bacteriology: isolation of bacteria from plant material, cultivation, microscopy, gas chromatography (GC), mass spectrometry (MALDI-TOF MS), Biolog identification system, molecular biological methods. Biological tests to confirm the phytopathogenicity of bacteria. 3. Diagnostic methods used in the Laboratory of Virology of the ÚKZÚZ: ELISA, biological tests, maintenance of a collection of reference material. 4. Diagnostic methods used in the Laboratory of Molecular Biology of the Institute of Molecular Biology of the Institute of Molecular Biology: polymerase chain reaction (PCR, qPCR, mPCR, nPCR, RT-PCR, PCR-RFLP), nucleic acid sequencing (DNA barcoding, NGS). 5. Introduction of new diagnostic tests in the laboratories of ÚKZÚZ. Resources and EPPO standards. Validation and verification of tests - General criteria and rules for individual groups of organisms, performance parameters, practical examples of validations molecular methods - factors affecting individual performance parameters. Examples of method validations in virology, bacteriology, mycology. 6. International projects and collaboration. European reference laboratories and national reference laboratories. Quality testing - Proficiency testing of laboratories (Proficiency tests)
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Homework for Teaching
- 10 hours per semester
- Attendace
- 13 hours per semester
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Learning outcomes
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Familiarization with diagnostic methods used in plant medicine and with real operation in a practical diagnostic laboratory.
Acquire knowledge related to the operation of a practical diagnostic laboratory focused on plant medicine.
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Prerequisites
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Knowledge of basic methods used in biochemistry and molecular biology.
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Assessment methods and criteria
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Student performance
Written test, 10 questions, minimum 70% pass rate required.
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Recommended literature
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Norma ČSN EN ISO/IEC 17025, Posuzování shody -- Všeobecné požadavky na způsobilosti zkušebních a kalibračních laboratoří..
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Alberts, B. a kol. (1998). Základy buněčné biologie. Espero Publishing, Ústí nad Labem.
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Alberts, B., Heald, R., Johnson A., Morgan, D., Roberts, K., Walter, P., Wilson, J., Hunt, T. (2022). Molecular biology of the cell (7. vydání).
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Bartlett, J.M.S, Stirling, D. (2003). PCR Protocols (second edition).
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Bustin, S. A. (2004). A-Z of Quantitative PCR.
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Filion, M. (2012). Quantitative Real-Time PCR in Applied Microbiology.
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Kůdela V., Novacky A., Fucikovsky L. (2002). Rostlinolékařská bakteriologie. Academia.
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Rosypal, S. (1996). Úvod do molekulární biologie. Brno.
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