| Course title | Practicals in Biochemistry |
|---|---|
| Course code | KBC/BCHC |
| Organizational form of instruction | Exercise |
| Level of course | Bachelor |
| Year of study | 2 |
| Semester | Summer |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory, Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
|---|
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| Course content |
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Identification of amino acids. Determination of the isoelectric point of casein. Detection of the presence of glutamic acid in food flavorings. Qualitative and quantitative analysis of carbohydrates in sweeteners. Determination of proteins by Bradford method in tube and microplate arrangement. Isolation of amine oxidase from pea seedlings and determination of its activity. Activity, substrate specificity and pH optimum of enzymes. Isolation of Ri-plasmid DNA from the bacterial strain Agrobacterium rhizogenes R15834 by alkaline lysis. Preparation of lipid fractions from egg yolk. Separation methods. Gel chromatography. Agarose electrophoresis. Determination of isoenzyme representation of plant peroxidases by discontinuous electrophoresis in polyacrylamide gel under native conditions.
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| Learning activities and teaching methods |
Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
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| Learning outcomes |
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Practical realization of basic experimental method from biochemisty.
Apply and practice fundamental biochemical procedures and methods, apply methods in the practice. |
| Prerequisites |
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Basic knowledge of biochemistry.
KBB/BZP ----- or ----- KFC/BZP |
| Assessment methods and criteria |
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Student performance
1) 100% participation in the exercise (possibility of replacing up to 2 absences for serious reasons, exceptional situations will be dealt with individually, e.g. hospital stay). Any absence must always be excused in writing (email) in advance before the start of the exercise. 2) Submitted and acknowledged protocols from all exercises. A pair of students solving a given task together will submit one joint protocol in electronic form. The prepared protocols must be submitted no later than the beginning of the following exercise (or within 7 days in the case of replacing the exercise and the last exercise)! For each protocol submitted on time and correctly prepared, the student will receive 5 points. In case of failure to meet the deadline for submitting the protocol, 5 points are deducted from the students. The protocol is corrected and scored after submission. The protocols are then no longer subject to further corrections. 3) During the exercise, the student's readiness will be tested in written form (5 questions from the theoretical and practical part of the task). Each correctly answered question is awarded 2 points. If the student receives 3 points or less, he/she will be expelled from the exercise and will have to repeat the exercise on an alternative date. In case of obtaining 4-5 points the supervisor is obliged to examine the student orally, in case of ignorance of the given topic the student is banned from the exercise and has to repeat the exercise in an alternative term. Due to unpreparedness it is possible to replace the exercise only once. 4) The student must complete all requirements for the course and obtain the required number of points for preparedness and submitted reports within two weeks of the last exercise. After two weeks from the end of the practicum, the points earned and fulfillment of the requirements for credit for this course, i.e. 100% participation in the practicum and all logs submitted and accepted, are evaluated. Criteria for scoring: 75 - 100% points - Credit is awarded (1st regular attempt). 50 - 75% points - Student will write a credit paper (this must be written for more than 70% of the points to be awarded credit, the credit paper can only be repeated once (2nd and 3rd regular attempts). < 50% points - Student will not be awarded credit, must re-enroll in the course the following year. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology - Specialization in Experimental Biology of Plants (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biology and Ecology (2020) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Nanomaterial Chemistry (2024) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Applied Chemistry (2025) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology - Specialization in Experimental Biology (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics (2022) | Category: Physics courses | 1 | Recommended year of study:1, Recommended semester: Summer |