| Course title | Practicals in Biochemistry |
|---|---|
| Course code | KBC/BCHC |
| Organizational form of instruction | Exercise |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory, Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Identification of amino acids. Determination of isoelectric point of casein. Detection of glutamic acid in food flavorings. Qualitative analysis of carbohydrates in milk. Qualitative and quantitative analysis of carbohydrates in sweeteners. Protein determination by Bradford method in tube and microplate arrangement. Isolation of amine oxidase from pea seedlings and determination of its activity. Determination of Michaelis constant of interaction of pea amine oxidase with putrescine and inhibitory constant of selected inhibitor. Activity, substrate specificity and pH optimum of enzymes. Isolation of Ri-plasmid DNA from Agrobacterium rhizogenes strain R15834 by alkaline lysis method. Preparation of lipid fractions from egg yolk. Separation methods. Gel chromatography. Agarose electrophoresis. Determination of isoenzyme content of plant peroxidase by the method of discontinuous polyacrylamide gel electrophoresis under native conditions.
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| Learning activities and teaching methods |
Laboratory Work
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| Learning outcomes |
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Practical realization of basic experimental method from biochemisty.
Apply and practice fundamental biochemical procedures and methods, apply methods in the practice. |
| Prerequisites |
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Basic knowledge of biochemistry.
KBB/BZP ----- or ----- KFC/BZP |
| Assessment methods and criteria |
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Student performance
Regular attending of the laboratory practicals. Successful performing of all experiments. In-time submission of complete reports containing evaluation of results. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biophysics (2022) | Category: Physics courses | 1 | Recommended year of study:1, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular Biophysics (2015) | Category: Physics courses | 1 | Recommended year of study:1, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology - Specialization in Experimental Biology (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology - Specialization in Experimental Biology of Plants (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Nanomaterial Chemistry (2021) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biology and Ecology (2020) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Applied Chemistry (2022) | Category: Chemistry courses | 3 | Recommended year of study:3, Recommended semester: Summer |