|
Lecturer(s)
|
|
|
|
Course content
|
History of the microscopy. Physical characteristics of the optics in the light microscopy. Basic components of the light microscope: objectives, ocular, light, numeric aperture and magnification. Working rules with microscope; drawing, measurement and calculation of the microscopical objects; image analysis. Advanced microscopic methods: dark field, phase contrast, polarization, interference, Nomarski Differential Interference Contrast (DIC). Fluorescence microscopy: mechanism, basic components of fluorescent microscope, fluorescent dyes, basic fluorescent methods, cytospectroscopy. Principles of the confocal microscopy, basic confocal methods; various way of improvement the microscope image quality. Various types of microscopes: stereomicroscope, inversion microscope, 3D microscopy, STM and ASM microscopes; microscope as the micromanipulator. Preparation of the microscopic slides: native, permanent, smearing, squash and cutting; fixation of the materials, microscopic dyes. Electron microscopy: mechanism of the TEM and SEM, preparing of the electronmicroscopic slides. <br>Excursions: Electronmicroscopes - TEM and SEM (RCPTM - Department of carbon nanostructures, UP Olomouc). Microscopic equipments of the scientific laboratory (ÚEB Olomouc); Confocal microscope (Department of Cell Biology, Centre of the Region Haná, Olomouc).
|
|
Learning activities and teaching methods
|
|
Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
|
|
Learning outcomes
|
Light and electron microscopy, basic and advanced microscopic methods. Preparation of the microscopic slides and their documentation.
Student is able to (after attending the course): - Describe principles of image appearance in light and electron microscope. - Describe detailed structure of light microscope. - Explain principles of contrast microscopy. - Draw, measure and count in the microscope. - Prepare various types of microscopic slide. - Describe the main types of electron microscope (TEM, SEM), and preparation of EM slides.
|
|
Prerequisites
|
unspecified
|
|
Assessment methods and criteria
|
Oral exam
70% test
|
|
Recommended literature
|
-
http://biologie.upol.cz/mikroskopie.
-
http://www.olympusmicro.com.
-
http://www.paru.cas.cz/lem/book/Podkap/3.0.html.
-
Habrová, V. (1990). Mikroskopická technika. SPN Praha.
-
Hejtmánek, M. Úvod do světelné mikroskopie. Skripta UP Olomouc, 2001..
-
Knoz, J., Opravilová, V. Základy mikroskopické techniky. Skripta MU Brno, 1992..
-
Matis, D. a kol. (2001). Mikroskopická technika. Faunima, Bratislava.
-
Paleček, J. (1996). Biologie buňky, I. Základy mikroskopické cytologie. UK, Vydavatelství Karolinum, Praha.
-
Razin S. E. Plant microtechnique and microscopy. Oxford University Press, Inc., 1999..
|