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Lecturer(s)
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Koubová Kateřina, Mgr.
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Čížková Kateřina, doc. Mgr. Ph.D.
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Course content
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Bases of Histochemistry and Immunohistochemistry. The course covers basic histochemical methods used for the detection of various chemical substances (e.g. lipids, iron, polysacharides and enzymes) and the principles these methods are based on. In addition, their practical use and importance for the understanding of chemical processes in human body will be discussed. Immunohistochemical techniques as a subset of histochemistry offer high specificity and sensitivity for the detection of intra- and extracellular components using a specific bond of antibody to antigen. The principle and practical application in research and diagnostics will be discussed. Upon completion, students will be equipped with knowledge needed for following subjects and particularly for pathology which takes advantage of these methods in diagnostics.
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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The course covers basic histochemical methods used for the detection of various chemical substances (e.g. lipids, iron, polysacharides and enzymes) and the principles these methods are based on. In addition, their practical use and importance for the understanding of chemical processes in human body will be discussed. Immunohistochemical techniques as a subset of histochemistry offer high specificity and sensitivity for the detection of intra- and extracellular components using a specific bond of antibody to antigen. The principle and practical application in research and diagnostics will be discussed.
Upon completion, students will be equipped with knowledge needed for following subjects and particularly for pathology which takes advantage of these methods in diagnostics.
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Prerequisites
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Knowledge from histology.
HIE/VAB11 and HIE/VAA12
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Assessment methods and criteria
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Essay
Attendance at seminars is compulsory.
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Recommended literature
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Kiernan, J. (2008). Histological and Histochemical Methods: Theory and Practice. Cold Spring Harbor Laboratory Press.
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Wick, M. R. (2008). Diagnostic Histochemistry. Cambridge University Press.
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