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Lecturer(s)
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Lebeda Aleš, prof. Ing. DrSc.
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Sedlářová Michaela, doc. RNDr. Ph.D.
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Course content
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The main aims are collecting samples in the field and documentation; determination of causal agents; methods of cultivation and inoculation; microscopical study of infection structures of fungal pathogens and pathological changes in plant anatomy; digital data processing, image analysis in phytopathology. 1. Diagnostics of plant diseases. Symptoms, microscopical methods, biochemical and molecular markers. Causal agents - focused on viruses, bacteria, oomycetes, fungi. 2. Isolation of pathogens from infected plant material. Methods of cultivation and inoculation. Collections of phytopatogenic microorganisms - Czech Republic and EU. 3. Testing of plant resistance to pathogens, or resistance of pathogens to compounds used in plant protection. 4. Pathological plant anatomy and physiology - methods of study. 5. Digital image processing, image analysis in phytopathology. 6. Automatic classification vs. trends in taxonomy of fungal pathogens. 7. Collections of phytopathogenic microorganisms in CR and EU
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration, Laboratory Work
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Learning outcomes
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The course introduces groups of phytopathogens spread in the Czech Republic.
After attending the course a student should be able to: - Compare cultivation and inoculation methods used for various groups of plant pathogens according to their integrity and life strategy - Analyse digital images in order to classify fungal pathogens - Calculate degree of pathogen sporulation, determine damage of host tissues - Compare host reaction to pathogen attac in laboratory and field conditions - Debate effects of plant protection practices on plant metabolism and crop yield
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Prerequisites
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Assessment methods and criteria
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Dialog, Seminar Work
1. Previous or concurrent attendance of the subjects plant physiology 2. Assay on given topic/digital data processing 3. Colloquial discussion
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Recommended literature
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Ellis, M.B., Ellis J.P. (1997). Microfungi on land plants.. Richmond Publishing.
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Hluchý, M., Ackermann, P., Zacharda, M., Bagar, M., Jetmarová, E., Vaněk, G. (1997). Obrazový atlas chorob a škůdců ovocných dřevin a révy vinné.. Biocont laboratory.
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Miranda, M., Lebeda, A. (2008). Mass Screening Techniques for Early Selection of Disease Resistance in Neglected Crops: A Critical Appraisal.. Springer Publishers.
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Sedlářová, M., Vašutová, M., Petrželová, I. a kol. Atlas houbových organizmů (http://botany.upol.cz/atlasy/system/index.html).
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Sedlářová, M., Vinter, V. Patologické změny rostlinných pletiv.
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