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Lecturer(s)
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Ondřej Vladan, doc. RNDr. Ph.D.
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Navrátilová Božena, RNDr. Ph.D.
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Šenkyřík Josef Baltazar, Ing.
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Král Daniel, Mgr.
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Course content
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Lectures (2-hour block) 1. Introductory lecture - getting to know the content of the lectures and practical exercises, division into groups for exercises. Biotechnology - classification, interdisciplinarity, from history to the present, plant "green" biotechnology in the Czech Republic. 2. The essence of plant explant cultures, methods (techniques preserving and techniques increasing genetic variability of plants) and their applications in plant breeding. 3. The use of plant biotechnology in agriculture, the pharmaceutical industry, gene pool conservation, and environmental protection. 4. Molecular biology methods in agricultural practice and research, their use in addressing new problems brought by current biotechnology and agricultural practice. Example cases related to issues of GMOs, herbicide- resistant weeds, and phytopathology. 5. The use of ionizing radiation in biotechnology and plant breeding. 6. The future of plant biotechnology. Evaluation of experiments and practical applications. Practices: 1a. Basics of plant explant cultures and their possible application in biology teaching or in extracurricular clubs at primary schools and in teaching or seminars at secondary schools. 1b. Work safety, preparation of media, sterilization of instruments, glassware, and other tools. 1c. Working with plant material, establishing an aseptic culture, cultivation, micropropagation method. 2a. Androgenesis - work under a binocular microscope, sterilization of plant material, application in practice and in teaching. 2b. Embryogenesis - application of methods within teaching, sterilization of plant material and transfer to an aseptic environment, work under a binocular microscope. 3. Evaluation of established experiments and discussion of experiments. Influence of plant material/genotype, origin of the explant, solutions to contamination, morphology and anatomy of plants under in vitro conditions.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration, Laboratory Work
- Attendace
- 26 hours per semester
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Learning outcomes
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The aim of the course is to build on students' basic subjects (general biology, plant physiology, genetics, ecology). Lectures and exercises focus on plant biotechnology and reflect progress in this scientific field not only in basic research but also in new application areas such as genetically modified organisms, including legislation on their cultivation and use, the application of plant biotechnology in agriculture, the pharmaceutical industry, gene bank preservation, and environmental protection. The course introduces students to new applications of explant cultures and molecular biology methods in practice, as well as incorporating the acquired knowledge into teaching biology at primary and secondary schools.
Knowledge - basic terms in area of plant biotechnologies using of plant biotechnologies in practice Student obtains experience working in an aseptic environment at selected primary explant culture methods used in the context of plant breeding - anther culture, embryocultures, micropropagation of plants. Learns about the equipment of laboratories dealing with GMO plants.
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Prerequisites
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Student is able to orientation in the issue of plant biotechnologies,methods, their practices and questions of genetic modified plants (benefits and disadvantages).
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Assessment methods and criteria
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Dialog
1. Attending exercises and evaluating experiments, own photo documentation. 2. Student presentation (5 min) on the topic of biotechnology.
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Recommended literature
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Heldman D.R., Wheler M.B., Hoover D.G. Encyclopedia of Biotechnology in agriculture and food..
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Kreuzer H., Massay A. (2005). Biology and biotechnology.. Washington.
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Procházka S. a kol. (1998). Fyziologie rostlin..
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Ram, A. (2010). Biotechnology: theory and practice. Jaipur.
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Smith J.E. (ed.). Biotechnology, 3rd Edition, Cambridge University Press 1998, ISBN 0-521-44911-1.
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Trigiano R.N., Gray D.J. (2005). Plant development and biotechnology..
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