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Lecturer(s)
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Ševčík Juraj, prof. RNDr. Ph.D.
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Course content
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Theory of electrophoretic separations. Introduction to computer modelling and experimental validation. Zone electrophoresis. Isotachophoresis. Isoelectric focusing. System peaks and their identification. Graphic models, separation diagrams. Special electrophoretic systems and methods (off- and on-line electrophoretic preconcentration techniques, separation of optical active compounds, DNA, peptides and oligosaccharides).
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 20 hours per semester
- Preparation for the Exam
- 80 hours per semester
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Learning outcomes
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Theory of electrophoretic separations. Introduction to computer modelling and experimental validation. Zone electrophoresis. Isotachophoresis. Isoelectric focusing. System peaks and their identification. Graphic models, separation diagrams. Special electrophoretic systems and methods (off- and on-line electrophoretic preconcentration techniques, separation of optical active compounds, DNA, peptides and oligosaccharides).
Compare advantages and disadvantages of particular electromigration methods and techniques, choose the suitable method for a given purpose, interpret results ( evaluation )
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Prerequisites
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Successfully graduation master's programme Analytical chemistry.
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Assessment methods and criteria
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Oral exam
To become acquainted with the subject matter according to guarantor or teacher recommendations and prove capability to explain and discuss given topic.
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Recommended literature
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Časopis Electrophoresis od r. 1990.
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Baker D.L. (1995). Capillary Electrophoresis. John Wiley, New York.
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Buszewski B. Dziubakiewicz E, Szumski M. (2013). Electromigration techniques, Theory and Practicle. springer, Berlín.
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Foret F., Křivánková L., Boček P. (1993). Capillary Electrophoresis (Radola B. J., ed.). VCH Verlagsgesellschaft Weinheim.
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Garcia CD., Chumbimoni-Tores K.Y., Carilho E. (2013). Capillary Electophoresis and Microchip Capillary electrophoresis, Principles. Yohn Wiley, New Jersey.
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Chrambach A., Dunn M.J., Radola B.J. (eds.). (1994). Advances in Electrophoresis 7. VCH Verlagsgesellschaft Weinheim.
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J. P. Landers. (2007). Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques. CRC Press.
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Rubinstein I. (ed). (1995). Physical electrochemistry: principles, methods, and applications. New York: Marcel Dekker.
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1. Dvořák J., Koryta J., Boháčková V. (1975). Elektrochemie. Academia, Praha.
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