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Lecturer(s)
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Fryčák Petr, doc. RNDr. Ph.D.
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Course content
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The students are taught the issue of therapeutic substances determination in bodily fluids for the purpose of therapy individualisation by appropriate dosage adjustment. The emphasis is put especially on drugs with narrow therapeutic ranges. The course comprises both an overview of most frequently used procedures for biological sample pretreatment, analyte separation from the sample matrix and methods for subsequent determination, and basic information on clinical aspects concerning usage of monitored drugs. Sample pretreatment techniques. Liquid-liquid extraction. Solid phase extraction. Microextraction techniques. Separation methods. Gas chromatography, GC/MS. Liquid chromatography, LC/MS. Capillary electrophoresis. Immunoanalysis. Basics of pharmacology, pharmacokinetics, ADME model, pharmacodynamics. Therapeutic drug monitoring of " cancer chemotherapeutics " antibiotics " antiepileptics " antidepressants and antipsychotics " immunosupresiv drugs Pharmacogenomics basics.
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Learning activities and teaching methods
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Lecture
- Attendace
- 26 hours per semester
- Homework for Teaching
- 13 hours per semester
- Preparation for the Course Credit
- 21 hours per semester
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Learning outcomes
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The aim of the course is to convey knowledge pertaining to therapeutic drug monitoring (a branch of clinical chemistry and pharmacology) that is concerned with monitoring of drug levels in bodily fluids for the purpose of drug therapy individualisation by appropriate dosage adjustment.
Comprehension of techniques for biological sample pretreatment and methods for separation and determination of drugs with respect to therapy individualisation by appropriate dosage adjustment. Understanding the mechanism of therapeutic effect of monitored drugs in relation to the corresponding pathology.
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Prerequisites
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Successfully completed study of chemistry at the B.S. level.
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Assessment methods and criteria
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Oral exam, Written exam
To demonstrate sufficient knowledge of the subject at the exam.
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Recommended literature
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Brozmanová H. Laboratorní metody v terapeutickém monitorování léků. Klinická farmakologie a farmacie. 2020;34(2):56?62..
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Češková E., Šilhán P., Kacířová I., Hýža M., Mayerová M., Grundmann M. Terapeutické monitorování léčiv v psychiatrii. In: Doporučené postupy psychiatrické péče 2018. Psychiatrická společnost ČLS JEP, 2018..
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Dasgupta A. (ed.). Therapeutic Drug Monitoring: Newer Drugs and Biomarkers. 2nd ed. Academic Press (Elsevier), 2024..
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Derendorf H., Schmidt S. Rowland and Tozer's Clinical Pharmacokinetics and Pharmacodynamics: Concepts and Applications. 5th ed. Wolters Kluwer, 2019..
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Grundmann M., Kacířová I. Terapeutické monitorování hladin antiepileptik I. ? obecné zásady, ?stará? antiepileptika. Klinická farmakologie a farmacie. 2016;30(2):9?15..
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Kacířová I., Grundmann M. Terapeutické monitorování hladin antiepileptik II. ? ?nová? antiepileptika, speciální skupiny pacientů. Klinická farmakologie a farmacie. 2016;30(2):16?22.
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Kacířová I., Grundmann M. Terapeutické monitorování léčiv ? review. Klinická farmakologie a farmacie. 2020;34(2):47?55..
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Suchánková H. Terapeutické monitorování koncentrací léčiv v klinické praxi. Praktické lékárenství. 2018;14(2):67?69..
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Švihovec J. a kol. Farmakologie. Praha: Grada Publishing, 2018..
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Tesfaye H. Terapeutické monitorování léčiv v současném pojetí. Remedia. 2014;24:52?57..
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