| Course title | Methods of Molecular Biology |
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| Course code | LRR/MMBIO |
| Organizational form of instruction | Lecture + Exercise |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Winter |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Lectures 1) PCR principle, PCR types, practical application 2) Primers, their design and use, work with sequences and databases 3) Recombinant technologies, restriction, ligation 4) Vector types, cloning 5) Nucleic acid isolation 6) Gene expression quantification I, transcription detection 7) Gene expression quantification II, expression determination 8) Transgenic organisms, transformation 9) Gene editing 10) Application of molecular genetics in breeding Exercises 1) Amplification of the gene of interest using PCR, primer design, work with databases, 4 hrs. (2/2) 2) Preparation of cloning vector, restriction, ligation, transformation into bacteria, 4 hrs. (2/2) 3) Preparation of expression vector, use of recombinant "Gateway" technologies, 4 hrs. (2/2) 4) RNA isolation, determination of concentration and quality, 4 hrs. (2/2) 5) Transcription into cDNA, sample preparation for RT-PCR, transcription detection, 4 hours (2/2) 6) Quantitative PCR of selected transgenes of genes, determination of copy number, 4 hours (2/2) 7) Colloquium, 2 hours
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| Learning activities and teaching methods |
| Laboratory Work |
| Learning outcomes |
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The course introduces students to basic and advanced methods of molecular biology and their practical use. The course focuses on modern laboratory techniques and procedures used to study nucleic acids (DNA, RNA) and gene expression. The course also includes information on methods of plant transformation, including gene editing and their application in genetics and breeding.
basic methods of molecular biology, preparation of cloning and expression vectors, RNA isolation, PCR, RT-PCR |
| Prerequisites |
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Application P-learning
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| Assessment methods and criteria |
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Student performance
During the course, students will learn theoretically and, most importantly, practically about molecular biology methods and their use in genetic engineering. They will learn how to design primers and amplify the gene of interest using PCR. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology - Specialization in Experimental Biology of Plants (2021) | Category: Biology courses | 3 | Recommended year of study:3, Recommended semester: Winter |