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Lecturer(s)
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Bieleszová Kristýna, Mgr.
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Žukauskaite Asta, Dr., Ph.D.
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Pěnčík Aleš, Mgr. Ph.D.
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Plačková Lenka, Mgr. Ph.D.
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Petřík Ivan, Mgr.
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Hrdlička Jakub, Mgr. Ph.D.
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Kaleta Michal, Mgr. Ph.D.
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Včelařová Ludmila, Mgr.
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Hladík Pavel, Mgr.
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Course content
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The laboratory safety, chemicals-storage, purity, basic chemical calculations, common buffers preparation instructions. Weighting and Scales: mechanical scales, digital scales, solid and liquid chemicals weighting. Volume and admeasurements determination: laboratory glass volume measurement, pipeting and various digital pipetes. Common laboratory equipment: thermostats, water baths, shakers etc. Potentiometry: equipment calibration, buffers, pH-measurement. Chromatography: basic principles, TLC. Excursion in the laboratory of Molecular and Cell biology.
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Learning activities and teaching methods
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Lecture, Laboratory Work
- Homework for Teaching
- 2 hours per semester
- Preparation for the Course Credit
- 6 hours per semester
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Learning outcomes
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The laboratory safety, chemicals, solutions, basic chemical calculations, common buffers preparation instructions.
Student should be able to (after attending the course): - work safely in the laboratory - prepare basic solutions and measure their physical characteristics - perform basic Spectrophotometry and Chromatography techniques
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Prerequisites
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unspecified
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Assessment methods and criteria
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Analysis of Activities ( Technical works), Systematic Observation of Student, Written exam
1) 100% presence at exercises 2) laboratory reports 3) test in extent of the lectures
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Recommended literature
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Eysseltová, J. a kol. (2000). Základy laboratorní techniky. Nakladatelství Karolinium Praha.
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Ferenčík, M. a kol. (1981). Biochemické laboratórne metódy, ALFA, Bratislava 1989. Alfa Bratislava.
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Ferenčík, M. a kol. (1981). Biochemické laboratórne metódy. Bratislava.
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Káš, J. a kol. (2000). Laboratorní cvičení z biochemie. Nakladatelství Olomouc.
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Kašpárek, F. a kol. (2000). Cvičení z laboratorní techniky. Vydavatelství UP Olomouc.
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Mikeš, V. (1992). Základní biochemické praktikum.. Brno.
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Mikeš, V. (1992). Základní biochemické praktikum. Vydavatelství MU Brno.
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Pazdera, P. a Potáček, M. (1984). Laboratorní cvičení z organické chemie. Vydavatelství UJEP Brno.
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Peč, P. a kol. (2000). Laboratorní cvičení z biochemie. Vydavatelství UP Olomouc.
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