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Lecturer(s)
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Oklešťková Jana, Mgr. Ph.D.
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Course content
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Immune system, immune response, immunoglobulins, molecular biologie of antibodies, immunochemicals methods, monoclonal, polyclonal and recombinant antibodies, antigen, haptens, methods of conjugation, immunozation, isolation and purification of antibodies; Monoclonal antibodies: hybridoma technologies, characteristic of splenocytes, myeloma cells and hybridomas, growing hybridomas, in vitro and in vivo production methods, immunoassays, biotechnologies, advantages and disadvantages of monoclonal, polyclonal and recombinat antibodies.
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Learning activities and teaching methods
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Lecture
- Homework for Teaching
- 15 hours per semester
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Learning outcomes
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To lern introduced section of hybridoma and recombinant technologies.
Student should be able to (after attending the course): - characterise the immune response; - recall the principles of immunochemicals methods; - explain the differences between monoclonal, polyclonal and recombinant antibodies; - describe and interpret hybridoma technologies; - judge the advantages and disadvantages of monoclonal, polyclonal and recombinat antibodies;
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Prerequisites
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Students should have a basic knowledge of immunology.
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Assessment methods and criteria
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Written exam
75% correctly answered questions in written test
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Recommended literature
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auct. (2007). Fundamental Techniques in Cell Culture: A Laboratory Handbook. Sigma-Aldrich.
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Ferenčík M. (1989). Imunochémia. Alfa, Bratislava.
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Harlow E., Lane D. (1988). Antibodies a laboratory manual. Cold Spring harbor Lab. USA.
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McCullough K., Spier R. E. (1990). Monoclonal antibodies in biotechnology: theoretical and practical aspects. Cambridge University Press, Cambridge, UK.
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Voet D., Voetová J. G. (1990). Biochemie. Victoria Publishing, Praha.
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