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Lecturer(s)
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Berka Karel, prof. RNDr. Ph.D.
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Hof Martin, prof. Dr. Ph.D.
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Anzenbacher Pavel, prof. RNDr. DrSc.
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Course content
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1. Spectrum-concept, fundamental characteristics and relations. Absorption spectra. 2. UV/VIS spectroscopy. 3. Absorption spectra and structure of substances. 4. IR spectroscopy. Introduction, basic vibrations. Instrumentation. 5. IR spectra-structure of substances. Raman spectroscopy-its use for the study of the structures of biological macromolecules. 7. Resonance Raman spectroscopy. 8. Mössbauer spectroscopy. Introduction into NMR and ESR. 9. NMR spectra-instrumentation and applications. ESR spectra. 10. Emission spectra. Fluorescence spectroscopy-instrumentation. 11. Steady-state and time-differentiated fluorescence. Examples of applications as to the studying the structure of substances and their interactions. 12. Fluorescence correlation spectroscopy (FCS), relaxation of solvate layers. 13. Chirooptical methods /ORD, CD/. The study of the structure of biological macromolecules.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Preparation for the Exam
- 0 hours per semester
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Learning outcomes
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The lectures are focused on advanced concepts from the field of spectroskopic methods.
ability to analyze and interpret the obtained spectra
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
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Recommended literature
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