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Lecturer(s)
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Course content
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Omics approaches ? metabolomics, genomics, proteomics. Targeted vs. non-targeted analysis. Metabolic fingerprinting. Typical instrumentations (LC-MS, GC-MS, NMR), description and specifications. High resolution MS. Design of metabolomic experiment. Priciple of differential analysis. Quantitative analysis of 3D chromatograms. Dimensionality reduction by fragment clustering. Accurate mass determination and elemental composition. Putative identification by using online databases. Correlation analysis and visualization. Transformation and standardization of data. Multivariate analysis (cluster and ordination analyses) and its interpretation. Candidates of active compounds. Plant and human metabolomics, sample preparation and common standards. Analysis of primary and secondary metabolites. Examples of metabolomic experiments. Biomarker discovery.
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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The knowledge of basic methods and work procedures in metabolomics.
Student will be able to propose experiment in metabolomics, select optimal analytical methods and proper method for data evaluation.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam, Written exam
70% fruitfulness on imposition
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Recommended literature
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K. Saito, R.A. Dixon, and L.Willmitzer. (2006). Plant Metabolomics.
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Nigel W. Hardy and Robert D. Hall. (2012). Plant Metabolomics.
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Wolfram Weckwerth. (2007). Metabolomics.
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