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Lecturer(s)
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Gonzalez Gabriel, Mgr. Ph.D.
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Kvasnicová Marie, Mgr. Ph.D.
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Jorda Radek, Mgr. Ph.D.
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Rárová Lucie, Mgr. Ph.D.
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Course content
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Working in sterile condition, cultivation and synchronization of mammalian cell cultures, counting of cells using Burker chamber, light and fluorescence microscopy, trypsinization of cells, harvesting, lysis and fixation of cells, gel electrophoresis in acrylamide gel, western blotting, detection of protein using chemiluminiscent detection, cell cycle analysis using flow cytometry, determination of intensity of GFP-fluorescence by fluorimetric reader
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Learning activities and teaching methods
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Observation, Demonstration, Laboratory Work
- Homework for Teaching
- 10 hours per semester
- Attendace
- 20 hours per semester
- Preparation for the Course Credit
- 20 hours per semester
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Learning outcomes
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Development, characterization, methods and testing in experimental biology.
Student should be able to (after attending the course) to use methods of experimental biology
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Prerequisites
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This course is intended exclusively for students studying the third year of the program who are expected to have the appropriate knowledge; successful completation of theoretical courses LRR/EXBPR, KBB/BUBIO, LRR/OBB, KBC/BCH, KBB/MBIO1 and practises LRR/ZCHV.
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Assessment methods and criteria
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Student performance, Written exam
100% attending of the laboratory practicals. Successful performing of all experiments. In-time submission of complete reports containing evaluation of results.
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Recommended literature
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