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Lecturer(s)
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Mistrík Martin, Mgr. Ph.D.
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Course content
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1. Introduction to Human and Animal Biotechnology - history, development, and classification of biotechnology - importance of biotechnology in medicine, agriculture, and industry 2. Biotechnological Methods and Experimental Approaches - molecular biology techniques - cell culture methods - sequencing technologies - bioinformatics and omics approaches 3. Molecular Cloning - recombinant DNA technology - expression systems - construction of expression vectors 4.Genome Editing and Genetic Engineering - CRISPR/Cas systems - TALENs - zinc-finger nucleases (ZFNs) - research and therapeutic applications 5.Transgenic Organisms - transgenic animals - knockout and knock-in models - applications in biomedical research and biopharmaceutical production 6. Reproductive Biotechnology - assisted reproduction - embryo transfer - cloning - reproductive technologies in livestock production 7. Cell Technologies - primary cell cultures - immortalized cell lines - organoids - three-dimensional cell culture models 8. Production of Biopharmaceuticals - recombinant proteins - enzymes - hormones - growth factors - industrial production systems 9. Therapeutic Antibodies and Targeted Biological Therapy - monoclonal and bispecific antibodies - antibody-drug conjugates (ADCs) - cancer immunotherapy 10. Gene and Cell Therapy - viral and non-viral vectors - CAR-T cell therapy - somatic cell genome editing - clinical applications 11. Stem Cell Technologies and Regenerative Medicine - embryonic and adult stem cells - induced pluripotent stem cells (iPSCs) - tissue engineering 12. Biotechnology in Vaccine and Drug Development - conventional and recombinant vaccines - mRNA vaccines - viral vector-based vaccines - personalized medicine 13. Ethical, Legislative, and Biosafety Aspects of Biotechnology - bioethics - GMO regulations - biosafety - potential misuse of biotechnology
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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The course aims to introduce students to the principles and current applications of human and animal biotechnology, with particular emphasis on their use in medicine, biomedical research, and animal production. The course covers molecular, cellular, and genetic technologies, their practical applications, technological limitations, as well as the ethical and societal issues associated with their implementation.
Upon successful completion of the course, students will be able to: - understand the major areas of modern human and animal biotechnology, - explain the principles of key biotechnological methods used in research, diagnostics, and therapy, - describe the principles of genetic engineering, production of biological therapeutics, and cell-based therapies, - evaluate the opportunities and limitations of current biotechnological applications, - critically assess the ethical, legislative, and biosafety aspects of biotechnology.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written exam
Successful oral exam.
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Recommended literature
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Bernard R. Glick, Cheryl L. Patten. (2022). Molecular Biotechnology - Principles and Applications of Recombinant DNA, 6th Edition. Washington, DC, USA, https://www.enbook.cz/.
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Hajdúch, Marián. (2000). Monoklonální protilátky v onkologii. Praha, https://www.linkos.cz/.
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Renneberg, Reinhard. (2023). Biotechnology for Beginners. Oxford, https://www.megaknihy.cz/.
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