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Lecturer(s)
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Šamajová Olga, Mgr. Dr.
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Krasylenko Yuliya, Ph.D.
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Hlaváčková Kateřina, Mgr. Ph.D.
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Maňáková Klára, Mgr.
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Course content
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Basic terminology, history, and principles of in vitro techniques. Principles of aseptic handling and cultivation of plant material. Nutrition of plant tissue cultures, including the composition of culture media and the use of plant growth regulators. Physical conditions of in vitro cultivation. Basic types of plant in vitro culture systems, including embryo, organ, tissue, cell, and protoplast cultures. Micropropagation. Totipotency of plant cells. Morphogenesis and its regulation. Organogenesis. Somatic embryogenesis. Haploid techniques, including androgenesis and gynogenesis. Plant regeneration. Somaclonal variation. In vitro conservation of plant genetic resources. Applications of plant tissue cultures in plant biotechnology. Laboratory Exercises Laboratory classes focus on the practical application of aseptic techniques for handling plant material, preparing culture media, establishing plant tissue cultures, and cultivating and propagating plants under in vitro conditions. Students will independently establish tissue cultures for somatic embryogenesis, perform successive subcultures to regenerate whole plants, and subsequently transfer the regenerated plants to ex vitro conditions. Individual developmental stages (callogenesis, embryogenesis, and plant regeneration) will be documented photographically and evaluated using appropriate statistical methods.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming), Observation, Demonstration, Laboratory Work
- Homework for Teaching
- 4 hours per semester
- Attendace
- 52 hours per semester
- Preparation for the Course Credit
- 4 hours per semester
- Preparation for the Exam
- 40 hours per semester
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Learning outcomes
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The course Plant Tissue Cultures aims to introduce students to the fundamentals of in vitro plant culture and its applications in plant biotechnology. Students will acquire theoretical knowledge of the principles underlying the establishment, cultivation, and maintenance of plant tissue cultures, while developing practical skills in aseptic handling of plant material, preparation of culture media, initiation of tissue cultures, and cultivation of plants under in vitro conditions. .
Students will gain an understanding of the fundamental methods of plant tissue culture and their applications in plant biotechnology. They will be able to perform aseptic manipulation of plant material, independently establish plant tissue cultures, and apply basic techniques for cultivating plants under in vitro conditions.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written exam, Written exam
Attendance at all practical classes (100%) and successful completion of the credit test.
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Recommended literature
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Clark, D.P. and Pazdernik, N.J. (2016). Biotechnology. Amsterdam.
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Loyola-Vargas V.M., Vázquez-Flota F. (2006). Plant cell culture protocols, 2-nd edition. Humana Press.
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Smith R.H. (2000). Plant tissue culture. Techniques and experiments, 2-nd edition. San Diego.
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Šamaj J., Bobák M., Ovečka M., Blehová A., Preťová A. (1997). Structural features of plant morphogenesis in vitro. Bratislava.
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Trigiano R.N, Gray D.J. (2011). Plant tissue culture, development and biotechnology. CRC Press.
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