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Lecturer(s)
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Course content
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1) Introduction and course overview. Genetic markers: morphological markers, biochemical markers, hybridization-based DNA markers. 2) Polymerase chain reaction (PCR)-based DNA markers. 3) Sequence-based DNA markers. Overview of methods used for DNA sequencing. 4) Single nucleotide polymorphism (SNP): characterization of new SNP loci, genotyping of known SNP loci. 5) Construction of recombination maps: types of mapping populations, genotyping of mapping populations, linkage analysis. 6) Mapping of quantitative trait loci (QTLs) and major-effect genes. Bulk segregant analysis (BSA) and selective genotyping. 7) Positional cloning of genes. 8) Marker-assisted selection (MAS). 9) Site-directed nucleases (SDNs): zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs). 10) CRISPR/Cas nucleases. Methods for detecting mutations introduced by SDNs. Use of SDNs in plant breeding. Legislation. 11) Targeting Induced Local Lesions IN Genomes (TILLING). In the seminar sessions, students are required to present recent original research and review papers. Each presentation is followed by a discussion. Credit is granted upon successful delivery of the seminar presentation.
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Learning activities and teaching methods
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Lecture, Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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Gaining insight into the principles and applications of molecular methods used in plant breeding.
Students will become familiar with the principles and applications of molecular methods used in plant breeding.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written exam
To pass the course, students must successfully complete a written exam with a minimum score of 18 out of 30 points. Course credit will be granted for a presentation delivered during the seminar.
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Recommended literature
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ACQUAAH, George. (2020). Principles of Plant Genetics and Breeding.
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Jain, S. M., & Brar, D. S. (2010). Molecular techniques in crop improvement. Dordrecht.
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Murphy, D. (2007). Plant Breeding and Biotechnology.
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MURPHY, Denis J. (2007). Plant Breeding and Biotechnology: Societal Context and the Future of Agriculture. Cambridge.
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SUZA, Walter a Kendall LAMKEY (eds.). (2023). Molecular Plant Breeding. Ames.
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WOODS, Kiara. (2023). Plant Breeding and Genetics: A Molecular Approach.
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Xu, Y. (2010). Molecular Plant Breeding. International Maize and Wheat Improvement Center (CIMMYT), CABI International.
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XU, Yunbi. (2010). Molecular plant breeding. Wallingford.
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