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Lecturer(s)
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Lazár Dušan, prof. RNDr. Ph.D.
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Course content
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<ul> <li> Types of luminescence, Jablonski diagram of organic molecules <li> Quantum-chemical methods of calculation of chlorophyll molecule and interpretation of absorption spectra <li> Fluorescence, phosphorescence and delayed light emissions of chlorophylls in the model systems <li> Fluorescence of chlorophyll a, b and carotenoids <li> Parameters of in vivo fluorescence <li> Emission and excitation spectra at different temperatures, their interpretation and usage <li> Fluorescence re-absorption and its quantification <li> Fluorescence transients (very fast, fast and slow fluorescence transients) <li> QB-reducing/QB-non-reducing, alpha/beta and other types of photosystem II centers <li> Fluorescence extinction - photochemical and non-photochemical extinction <li> Parameters determined from fluorescence induction curves and from induction of extinction and their interpretation <li> Fluorescence temperature curve, its phase, parameters and their interpretations <li> Fluorescence kinetics in ps- and ns-time range, lifetime <li> Polarization and coherence of chlorophyll fluorescence <li> Experimental methods of measurements of particular parameters of chlorophyll fluorescence </
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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<ul> <li> Types of luminescence, fluorescence, experimental methods of measurements of particular parameters of chlorophyll fluorescence </ul>
Evaluation Evaluate the particular methods and principles, explain the aspects and results concerning the given issue, integrate the knowledge, predict the solutions, evaluate the results and outcomes.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Mark
<ul> <li> Knowledge within the scope of the course topics (examination) </ul>
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Recommended literature
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Amesz, J., Hoff, A. J. (1996). Biophysical Techniques in Photosynthesis. Kluwer Academic Publishers, Dordrecht, Boston, London.
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Govindjee. (1995). Sixty-three Years Since Kautsky: Chlorophyll a fluorescence. Aust. J. Plant Physiol. 22: 131-160.
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Kaplanová M., Nauš J., Vacek K. (1987). Fyzikální základy fotosyntézy. SPN, Praha.
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Papageorgiou C. C., Govindjee (ed.). (2004). Chlorophyll a Fluorescence. A signature of Photosynthesis. Springer, Dordrecht.
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