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Lecturer(s)
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Lazár Dušan, prof. RNDr. Ph.D.
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Prasad Ankush, Mgr. Ph.D.
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Herbst Christian, MgA. Ph.D.
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Nauš Jan, prof. RNDr. CSc.
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Ilík Petr, prof. RNDr. Ph.D.
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Pospíšil Pavel, prof. RNDr. Ph.D.
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Brabec Viktor, prof. RNDr. DrSc.
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Kašpárková Jana, prof. RNDr. Ph.D.
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Švec Jan, prof. RNDr. Ph.D. et Ph.D.
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Kubala Martin, doc. RNDr. Ph.D.
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Course content
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Native and denaturation electrophoresis of proteins and pigment-protein complexes Gel-scanners and imaging HPLC of photosynthetic pigments EPR, electron spin echo, optically detected magnetic resonance, ENDOR Linear and circular dichroism FTIR and resonance Raman spectroscopy Photoacoustic spectroscopy Gasometric methods Kinetics of luminescence, thermoluminescence and chemiluminescence. AFM, STM and near field microscopy Optical scissors, micromanipulation with molecules Fluorescence microscopy and imaging methods Experimental methods for examination and analysis of voice: Laryngoscopic methods (laryngoscopy, laryngostroboscopy, high-speed videolaryngoscopy, videokymography), physiologic methods (electroglottography, photoglottography, pneumography, pneumotachography, measurements of subglottal pressure), acoustic methods (microphones and recording, acoustic spectrography, voice range profile, MDVP, DSI). Measurement of vocal load (voice dosimetry, time dose, cycle dose, distance dose, radiated energy dose, dissipated energy dose, the use of accelerometers for voice measurement).
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Learning activities and teaching methods
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Lecture
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Learning outcomes
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Basic knowledge in experimental methods of biophysics.
Evaluation Evaluate the particular methods and principles, explain the aspects and results concerning the given issue, integrate the knowledge, predict the solutions, evaluate the results and outcomes.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Mark
Knowledge within the scope of the course topics (examination).
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Recommended literature
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Vybrané časopisecké publikace.
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Amesz J., Hoff A. J.(ed.). (1996). Biophysical Techniques in Photosynthesis. Kluwer Academic Publishers, Dordrecht, Boston, London.
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Baken, R. J., Orlikoff, R. F. (2000). Clinical measurement of speech and voice. San Diego, CA: Singular Publishing Group.
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García-Campana A. M., Bayens W. R. G. (2001). Chemiluminescence in Analytical Chemistry. Marcel Dekker, Inc. New York, Basel.
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Isenberg G.(ed.). (1998). Modern Optics, Electronics and High Precision Techniques in Cell Biology. Springer, Berlin.
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Lakowitz, J. R. (1999). Principles of fluorescence spectroscopy (2.ed). Kluwer Academic/Plenum Publishers, New York.
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Prosser, V. a kol. (1989). Experimentální metody biofyziky. Academia Praha.
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Švec, J. (1996). Studium mechanicko-akustických vlastností lidského hlasu. PřF UP Olomouc.
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Valeur, B. (2006). Molecular fluorescence. Wiley-VCH, Weinheim.
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