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Lecturer(s)
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Danihlík Jiří, Mgr. Ph.D.
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Sekaninová Jana, Mgr. Ph.D.
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Zalabák David, Mgr. Ph.D.
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Dostálková Silvie, Mgr. Ph.D.
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Janků Martina, Mgr. Ph.D.
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Jemelková Jana, Mgr. Ph.D.
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Hrtánková Eva, Mgr.
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Jedelská Tereza, Mgr. Ph.D.
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Vrobel Ondřej, Mgr. Ph.D.
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Course content
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Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) followed by western blotting. Immunochemical detection of proteins transferred on PVDF membrane. Analysis of sugars using HPLC. Analysis of biogenic amines using liquid chromatography coupled with mass spectrometry. Light microscopy. Isoelectric focusing. Bioinformatic approach to protein analysis. PCR - identification of transformed bacterial colonies. Enzyme kinetics.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
- Homework for Teaching
- 100 hours per semester
- Attendace
- 104 hours per semester
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Learning outcomes
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Students perform practical experiments with the aim to acquire skills in advanced separation and instrumental methods that are used in biochemical laboratories.
To apply and practice fundamental biochemical procedures and methods.
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Prerequisites
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Students are expected to have the knowledge and skills obtained from successful completion of the following courses and laboratory practicals: KBC/BCH Fundamentals of Biochemistry, KBC/BIME Biochemical Methods and KBC/BCHC Laboratory practicals in Biochemistry.
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Assessment methods and criteria
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Student performance, Systematic Observation of Student
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Recommended literature
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Katoch, R. (2011). Analytical techniques in biochemistry and molecular biology. Springer, New York.
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Králová, B. (2001). Bioanalytické metody. VŠChT.
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Nováková, L., Douša, M. a kol. (2013). Moderní HPLC separce v teorii a praxi I. Europrint, 2013.
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Nováková, L., Douša, M. a kol. (2013). Moderní HPLC separce v teorii a praxi II. Europrint, 2013.
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Pingoud, A., Urbanke, C., Hoggett J., Jeltsch, A. (2002). Biochemical Methods: A Concise Guide for Students and Researchers. Weinheim.
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Pingoud, A., Urbanke, C., Hoggett J., Jeltsch, A. (2002). Biochemical Methods, A Concise Guide for Students and Researchers. Wiley-VCH, Weinheim.
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Šebela, M. a kol. (2016). Experimentální metody biochemie (elektronická skripta). Olomouc.
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Vashist S., Luong J. (2018). Handbook of Immunoassay Technologies 1st Edition. Academic Press.
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