| Course title | Practicals in Biochemistry |
|---|---|
| Course code | KBC/BCHCR |
| Organizational form of instruction | Exercise |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 5 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Identification of amino acids. Determination of the isoelectric point of casein. Detection of the presence of glutamic acid in food flavorings. Qualitative and quantitative analysis of carbohydrates in sweeteners. Determination of proteins by Bradford method in tube and microplate arrangement. Isolation of amine oxidase from pea seedlings and determination of its activity. Activity, substrate specificity and pH optimum of enzymes. Isolation of Ri-plasmid DNA from the bacterial strain Agrobacterium rhizogenes R15834 by alkaline lysis. Preparation of lipid fractions from egg yolk. Separation methods. Gel chromatography. Agarose electrophoresis. Determination of isoenzyme representation of plant peroxidases by discontinuous electrophoresis in polyacrylamide gel under native conditions.
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| Learning activities and teaching methods |
Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
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| Learning outcomes |
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Practical realization of basic experimental method from biochemisty.
Apply and practice fundamental biochemical procedures and methods, apply methods in the practice. |
| Prerequisites |
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Basic knowledge of biochemistry.
KBB/BZP ----- or ----- KFC/BZP |
| Assessment methods and criteria |
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Student performance
1. Participation in exercises - 100% attendance at exercises is mandatory. - In case of illness or other serious reasons, it is possible to replace one exercise with another group, but no more than once per semester. - Exceptional situations (e.g., hospitalization) will be dealt with individually. 2. Reports Preparation of reports - Pairs of students working on a given task submit one joint report in electronic form (.docx). - If one of the students fails to fulfill their obligations during the exercise, the instructor (or the student themselves) may decide that each student will submit their reports separately. Submission deadlines - The protocol must be submitted no later than the beginning of the next exercise. - If the exercise is completed on an alternative date, the report must be submitted no later than 7 days after completion of the task. - The report from the last exercise must also be submitted no later than 7 days after completion of the task. - All reports must be approved no later than 4 weeks after the date of the last exercise. Protocol evaluation - The maximum number of points for one protocol is 10 points. - The first submitted protocol is evaluated according to the severity of errors and deficiencies (full points are not automatic). - If the student does not submit the protocol by the deadline, 5 points are deducted. Protocol corrections - Each protocol may be returned for correction only once. - If the correction is not submitted within 7 days of the protocol being returned by the teacher, 5 points will be deducted. - If not all errors or deficiencies are corrected in the revised protocol, 2 points will be deducted. Plagiarism - If it is found that the protocol has been copied (e.g., the results do not correspond to the data in the workbook), the exercise is invalid and the student cannot receive credit. 3. Checking readiness for the exercise - During the exercise, the student's readiness will be tested. - The maximum number of points from the test is 6 points. Test evaluation - 2-3 points from the test: The student is tested orally by the exercise leader. o If they do not demonstrate knowledge of the topic, they are expelled from the exercise. o If they are familiar with the topic and only did not do well on the test questions, they continue with the exercise and their points are retained. - Less than 2 points on the test: The student does not continue with the exercise and must make it up. Due to lack of preparation, it is only possible to repeat the exercise once per semester. Specific cases - Students who attend fewer exercises due to public holidays, planned internships, or excursions, as well as third-year students who complete their studies earlier, will receive the corresponding number of points according to the applicable rules. AWARDING OF CREDIT To be awarded credit, students must: - meet all of the above requirements, - obtain a sufficient number of points for preparedness and submitted reports. Evaluation: - 75-100% of points credit is awarded. - 50-75% of points the student takes a credit test. The test must be completed with more than 70% of points. The credit test can only be repeated once. - less than 50% of points credit will not be awarded and the student must re-enroll in the course in the following academic year. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Biochemistry (2022) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Bioinorganic Chemistry (2021) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Chemistry (2019) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biotechnology and Genetic Engineering (2022) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Bioorganic Chemistry and Chemical Biology (2020) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Chemistry - Analytical Specialist (2021) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |