|
Lecturer(s)
|
-
Vrzalová Aneta, Mgr. Ph.D.
|
|
Course content
|
Introduction. In vitro translation, antibody immobilization, co-immunoprecipitation, SDS-PAGE, Western blot, immunodetection, evaluation of results.
|
|
Learning activities and teaching methods
|
|
Laboratory Work
|
|
Learning outcomes
|
Basic methods for studies of cellular processes (protein-protein interactions, interaction with DNA, postranslation modifications). In practice, students are introduced to co-immunoprecipitation and in vitro translation of functional recombinant proteins.
Student is able to (after attending the course): - understand of presented methods and apply them in practise - determine, evaluate and discuss the results.
|
|
Prerequisites
|
unspecified
|
|
Assessment methods and criteria
|
Student performance
Knowledge in the range of topics discussed, 100% participation, protocol development.
|
|
Recommended literature
|
-
Alberts, B. a kol. (2002). Molecular Biology of the Cell, 4th ed.. Garland Science, New York.
-
Cooper G. M. (1997). The cell. A molecular approach. ASM Press, Washington DC, USA.
-
JANSEN, J.C., RYDÉN, L. Protein Purification, Wiley-VCH, Weinheim, 1998..
|