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Lecturer(s)
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Šafářová Dana, Mgr. Ph.D.
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Nádvorník Petr, RNDr. Ph.D.
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Grycová Aneta, Mgr. Ph.D.
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Course content
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1) The course introduction. Laboratory safety and rules. 2) Isolation of Plant genomic DNA. Measuring of the DNA concentration and purity, its electrophoretic separation on agarose gel. 3) Isolation of imunoglobulin fraction IgG of rabbit blood serum. Electrophoretic separation of proteins on polyacrylamide gel (PAGE), detection of their molecular weights. 4) Plasmid transformation of competent bacteries, gene transfer of resistence to antibiotics. 5) Detection of aryl hydrocarbon receptor in tumor cell lines (protein izolation, SDS-PAGE, westernblotting, immunodetection).
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration, Laboratory Work
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Learning outcomes
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Basic metods of molecular biology, DNA and proteins izolation and their analysis, bacterial transformation.
Student is able to (after attending the course): - Apply the laboratory skills in molecular biology research. - Perform the basic molecular techniques. - Write properly research reports aimed at solving the given problems and discuss the results
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Prerequisites
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unspecified
KBB/BZP
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Assessment methods and criteria
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Student performance
100% attendance, protocols of practics, credit test
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Recommended literature
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Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2000). Základy buněčné biologie. Espero Publishing, Ústi nad Labem.
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Navrátil, M., Kubaláková, M., Kučerová, L. Sylabus cvičení z molekulární biologie doplněný návody.
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Nečas, O. a kol. (2000). Obecná biologie pro lékařské fakulty. H&H, Jinočany, 554 str.
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Raclavský, V. (1998). Úvod do základních metod molekulární genetiky. Skriptum LF UP v Olomouci.
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