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Lecturer(s)
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Divoký Vladimír, doc. RNDr. Ph.D.
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Hajdúch Marián, doc. MUDr. Ph.D.
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Course content
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Model systems (yiests, Drosophila, mice). PCR-based diganostic methods. Stem cells technology. Gene transfer into mammalian cells. Gene engineering in mammalina embryonal stem cells, gene knock-out, gene knock-in, nuclear transfer ("clonning"), CGH, Array CGH, M-FISH and other techniques of molecular cytogenetics. Gene libraries, preparation of genetic primers for diagnosis. Microdissection and its use in characterisation of each cell, linear DNA/RNA amplification. Microchips and its use for study of RNA expression or detection of SNPs. Analyses of proteins in Medicine (Western Blot, enzyme immunoanalysis, RIA, immunohistochemistry, immunofluorescence, Proteomics). Flow-cytometry. Monoclonal antibodies, technoogy of preparation, humanization, diagnostic and therapeutic utilization.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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Model systems (yiests, Drosophila, mice). Modern techniques and technologies used for their analysis and theri applicoation for diagnostic and terapeutic purposes.
Student should be able to (after attending the course): - Describe the model systems (yeasts, Drosophila, mice) - Describe and explain various modern diagnostic methods.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
oral exam in extent of the lectures
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Recommended literature
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Dale JW., Schantz M. From Genes to Genomes : Concepts and Applications of DNA Technology. Wiley Sons Ltd., 2002..
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Hajdúch, M., Michálek, J., Papajík, T., Štěrba, J., Talač, R., Vyzula, R., Žaloudík, J. (2000). Monoklonální protilátky v onkologii. Mediforum 2000, Maxdorf s.r.o., Praha.
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Jarošová M. Cytogenetika. Metody molekulární cytogenetiky. Onkocytogenetika. V: Hematologie pro zdravotní laboranty, Lexová S. a kol., str. 144-183. Vydavatelství IDVPZ, Brno, 2000..
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Kovářová H., Hajdúch M., Livingstone M., Džubák P., Lefkovits I. Analysis of state-specific phosphorylation of protein by two-dimensional gel electrophoresis. Journal of Chromatography B 787 (1): 53-61, 2003..
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Vachtenheim J., Duchoň J. Molekulární biologie pro mediky a lékaře. Karolinum Praha, 1992..
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