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Lecturer(s)
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Vrzal Radim, doc. Ing. Ph.D.
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Course content
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DNA, RNA and protein isolation, DNA quantification, Polymerase chain reaction (PCR), Souther blotting, Norther blotting, DNA sequencing, Western blotting, DNA typing - microsatelites, Gene cloning, Forensic applications - SNP, X-chromosom, mitochondrial DNA, Y-chromosom, protein profiling. Culprit´s identification by DNA fingerprinting
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Learning activities and teaching methods
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Lecture, Laboratory Work
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Learning outcomes
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To gain awareness about possibilities of biological material / individual identification by using DNA fingerprint.
Student is able to (after attending the course): - know and describe selected analytical methods used in molecular biology including their forensic applications - perform an identification of individual based on DNA profile
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written exam
- test (70%) - 100% atendance at practical lecture, protocol elaboration
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Recommended literature
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Carracedo A. (2005). Forensic DNA typing protocols (series: Methods in Molecular Biology, vol. 297). Tottowa, NJ, USA.
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Lincoln P.J., Thomson J. (1998). Forensic DNA profiling protocols (series: Methods in molecular biology, Volume 98).
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Rapley R., Whitehouse D. (2007). Molecular Forensics.
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