|
Lecturer(s)
|
-
Vrzal Radim, doc. Ing. Ph.D.
|
|
Course content
|
1. Safety in laboratory 2. DNA isolation from buccal mucous membrane 3. Measurement of isolated DNA concentration 4. Allele-specific PCR 5. Results analysis 6. Examples of inheritance from pedigrees
|
|
Learning activities and teaching methods
|
|
Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work
|
|
Learning outcomes
|
SNP variants analysis and heredity coming from pedigree
Student is able to (after attending the course): - Apply the laboratory skills in human genetics research. - Write properly research reports aimed at solving the given problems and discuss the results
|
|
Prerequisites
|
Basic knowledge of genetics
|
|
Assessment methods and criteria
|
Written exam, Student performance
100% attendance, protocol elaboration (acceptable quality) , test (70% correctly answered questions)
|
|
Recommended literature
|
-
Brdička, R. Lidský genom na rozhraní tisíciletí. Grada Publishing, Praha, 2001.
-
Bryšová V. a kol. Základy klinické genetiky. MU, Brno, 1995.
-
Kapras, J. et al. (1996). Kapitoly z lékařské biologie a genetiky. Praha, UK Karolinum.
-
Mueller, F.M., Young, I.D. (2001). Emeryś Elements of Medical Genetics. Churchill Livingstone.
-
Schaaf, Christian P. (2012). Human Genetics: From molecule to medicine. Lippincott Wiliams and Wilkins, Baltimore.
-
Sršeň, Sršňová. Základy klinickej genetiky. Osveta, Martin.
-
Thompson, J. and Thompson, M. (2001). Genetics in Medicine. W.B. Saunders Company.
|