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Lecturer(s)
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Šafářová Dana, Mgr. Ph.D.
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Hřibová Eva, Mgr. Ph.D.
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Nádvorník Petr, RNDr. Ph.D.
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Navrátil Milan, prof. RNDr. CSc.
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Course content
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Izolation of bacterial recombinant plazmid. Polymerase chain reaction (PCR) - aster yellows phytoplasma detection . Random fragment lenght polymorphism (RFLP) and gel electrophoresis in agarose gel. Analysis of microsatelite DNA locus polymorphisms. PCR In situ (PRINS) - physical localization of repetitive sequences on chromosomes. Fluorescence in situ hybridization (FISH) - physical localization of chromosome repetitive sequences.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work
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Learning outcomes
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Student should be able to (after attending the course): - isolate recombinant plasmids using ´spin´ tubes. - run a Polymerase Chain Reaction. - perform RFLP analysis of the PCR product. - perform in situ PCR on isolated chromosomes. - detect polymorphism of microsatellite loci. - analyse results and write a report (about aims, methods and results of the experiments).
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Prerequisites
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unspecified
KBB/BZP
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Assessment methods and criteria
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Student performance
attendance of practicals, protocols elaboration, 70% test
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Recommended literature
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Brown T.A. (2008). Klonování genů a analýza DNA. Nakladatelství UP Olomouc.
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Raclavský V. Metody molekulární genetiky.
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Šmarda, J., Doškař, J., Pantůček, R., Růžičková, V., & Koptíková, J. (2005). Metody molekulární biologie. Brno: Masarykova univerzita.
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Turňa I., Stuchlík S., Drahovská H., Gálová Z., Timko J. (2004). Techniky rekombinantních DNA. Veda.
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