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Lecturer(s)
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Hrubý Jiří, Mgr.
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Illés Peter, RNDr. Ph.D.
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Course content
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Working with a light microscope. Phospholipid auto-aggregation, biomembrane. Plasma membrane of erythrocyte. Cell fusion. Osmosis. Animal cell. Plant cell. Cell nucleus. Fluorescence microscopy - principle and application in cell biology. Cytochemical localization of cell structures. Cell separation, cell counting. Tests of cell viability. Cell survival in low temperatures. Cell cryopreservation.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Observation, Laboratory Work
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Learning outcomes
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Working with a light and fluorescent microscope, vizualization of cell components.
Student is able to (after attending the course): - work with light microscope; - work in the laboratory; - perform simple experiments with eukaryotic cells, to analyze and to interpret obtained data; - write reports about the experiments and to discuss the results; - define the common strategies of cell analysis.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Student performance, Didactic Test
attendance, protocols´ elaboration, 70% test
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Recommended literature
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Habrová V. Mikroskopická technika. SPN, Praha, 1990..
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Paleček J. Biologie buňky I. Základy mikroskopické cytologie. UK, Vydavatelství Karolinum, Praha, 1996..
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Razin S. E. Plant microtechnique and microscopy. Oxford University Press, Inc., 1999..
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