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Lecturer(s)
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Kitner Miloslav, RNDr. Ph.D.
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Majeský Luboš, RNDr. Ph.D.
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Course content
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The course is focused on practical mastery of basic molecular genetic techniques used in plant biosystematics. After completing this course, the student should be able to independently perform DNA isolation, verify its quality and quantity, perform PCR reactions and be able to process the results of Sanger sequencing. - Methods of molecular markers used for the study of plant biosystematics. Their overview, use, advantages and disadvantages. - Processing and working with sequencing data, working with databases, working with common statistical SW and applications.
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Learning activities and teaching methods
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Lecture, Work Activities
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Learning outcomes
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The course focuses on practical mastery of basic molecular biology techniques that are currently used in population genetic and phylogenetic studies of plants: DNA isolation, PCR techniques, separation and detection of PCR fragments, preparation of samples for the sequencing laboratory, evaluation of electrophoretograms, work with databases and statistical programs.
After completing the course, the student should be able to: - perform DNA isolation from plant material - routinely perform PCR reactions - optimize PCR reaction conditions - edit sequencing data, export the resulting data and construct a phylogenetic tree
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
oral exam: three questions a) general characteristics of DNA structure and its biological functions, PCR reaction b) principles of molecular markers techniques c) overview of the use of molecular markers in plant biosystematics
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Recommended literature
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Chaloupek O. 2000. Genetická diverzita, šlechtění a semenářství. Academia, Praha, 311 pp..
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Mazura I., Michalová K., Brdička R., Mácha J. 2001. Speciální metody molekulární biologie. Univerzita Karlova v Praze, Karolinum, 101pp..
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Vaculná L., Majeský Ľ., Kobrlová L., Kitner M. Záchrana zelených pokladů: využití metod populační genomiky v ochraně kriticky ohrožených druhů rostlin. Praha. 2024.
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Weising K., Nybom H., Wolff K., Kahl G. 2005. DNA fingerprinting in plants. Principles, methods and applications (2nd ed). CRC Press, Boca Raton, FL, USA, 444pp..
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