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Lecturer(s)
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Jirovský David, doc. RNDr. Ph.D.
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Milde David, doc. Ing. Ph.D.
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Papoušková Barbora, RNDr. Ph.D.
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Course content
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Atomic absorption spectrometry (AAS , atomic fluorescence spectrometry ( AFC), Optical emission spectrometry (OES- plasma spectrometry and mass spectrometry (MS) of organic molecules and inorganic ions IPS /MS) (principles, theories, instrumentation and application). Gas (GC) and liquid (LC,HPLC) chromatography (theory, instrumentation, sampling, columns, detectors, quality, quantity, GC,LC/MS). Electromigration (capillary electrophoresis and isotachophoresis ). Information on methods .
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Learning activities and teaching methods
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Lecture, Demonstration
- Attendace
- 39 hours per semester
- Homework for Teaching
- 13 hours per semester
- Preparation for the Course Credit
- 10 hours per semester
- Preparation for the Exam
- 28 hours per semester
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Learning outcomes
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The course provides the introduction to the separation, electromigration and some spectral methods.
Differentiate between particular analytical methods and techniques, explain principles, select typical methods for a given purpose
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Prerequisites
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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Assessment methods and criteria
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Mark, Written exam
100% attendance in seminars, Oral examination- right answers to questions both from spectral and separation methods
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Recommended literature
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Analytical Atomic Spectrometry with Flames and Plasmas , J.A.C. Broekaert, Wiley- VCH Verlag GmbH 2002.
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Atomová spektroskopie- T. Černohorský, P. Jandera, Univerzita Pardubice 1997.
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Churáček J. a kol.: Analytická separace látek, SNTL Praha, 1990..
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Modern Analytical Chemistry, D. Harvey, The Mc Graw Hill 2000.
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Principles of Instrumental Analysis, D.A.Skoog, F.J. Holler, T.A. Nieman , Brooks/cole Thomson Learning 1997.
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Štulík K. a kol: Analytické separační metody, Karolinum UK Praha 2004.
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