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Lecturer(s)
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Barták Petr, prof. RNDr. Ph.D.
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Course content
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Supercritical point and its surrounding, supercritical liquids and their physical properties ( density, viscosity, diffusivity, solubility ). Chemical properties of supercritical liquids, reaction in supercritical medium. Theoretical and practical aspects of supercritical fluid extraction and its influencing ( elution essence, solubility, modificators, derivatization, temperature and pressure influences ). Instrumentation SFE ( extraction cells, restrictors, capture ). SFE application ( both industry and laboratory ). Supercritical fluid chromatography SFC ( comparison with GC and HPLC, instrumentation, columns, restrictors, detectors, application ).
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Learning activities and teaching methods
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Lecture
- Attendace
- 13 hours per semester
- Homework for Teaching
- 13 hours per semester
- Preparation for the Course Credit
- 34 hours per semester
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Learning outcomes
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The aim is to get an overview of supercritical extraction and chromatography.
Define main concepts, describe main approaches in the field of separation techniques using supercritical fluids
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Prerequisites
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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Assessment methods and criteria
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Oral exam
Colloquium- discussion about given problems
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Recommended literature
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Analytical Supercritical Fluid Extraction : M.D.L. De Castro, M. Valcárel, M.T. Tena, Springer-Verlag, Berlin Heideberg 1994.
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L. Nováková, M. Douša a kol.: Moderní HPLC separace v teorii a praxi I, II. Praha 2013..
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L.M. Miller, J.D. Pinkston, L.T. Taylor: Modern supercritical fluid chromatography: Carbon dioxide containing mobile phases. John Wiley & Sons, Inc., Hoboken 2020..
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T.L. Chester, J.D. Pinkston, D.E. Raynie: Anal. Chem. 70, (1998) 301R-319R.
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T.L. Chester, J.D. Pinkston, D.E. Raynie: Supercritical fluid chromatography and extraction. Anal. Chem. 70 (1998) 301R-319R..
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