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Lecturer(s)
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Lemr Karel, prof. RNDr. Ph.D.
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Course content
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The aim is to provide deeper insight into principles and applications of mass spectrometry, information concerning new knowledge and to develop student´s abilities to solve research tasks and routine applications of mass spectrometry. Attention is focused on theory describing behavior of analytes in the course of mass spectrometric analysis (ionization of analytes, separation of ions, their fragmentation), on instruments (ion sources, mass analyzers, ion detection, vacuum systems) and data interpretation. Student acquaints with new procedures and techniques in mass spectrometric analysis (e.g. ambient ionization, mass spectrometry imaging, development of mass analyzers, ion mobility-mass spectrometry, miniaturization of mass spectrometers), with application of mass spectrometry in various fields, e.g. drug development, metabolomics, biomarkers, clinical applications, food analysis, forensic and security applications, analysis of art objects
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 20 hours per semester
- Preparation for the Exam
- 80 hours per semester
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Learning outcomes
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To offer students more detail insight to principles and application possibilities of mass spectrometry, information concerning new knowledge and to develop their skills to solve research as well as routine tasks using mass spectrometry.
To explain actual trends in mass spectrometry, on the basis of deeper knowledge of basic principles to choose a suitable procedure for a mass spectrometric analysis and to interpret obtained data.
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Prerequisites
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Successfully graduation master's programme Analytical chemistry
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Assessment methods and criteria
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Oral exam
To become acquainted with the subject matter according to guarantor or teacher recommendations and prove capability to explain and discuss given topic.
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Recommended literature
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Přehledové články věnované novým poznatkům v teorii, novým trendům v instrumentaci a zajímavým aplikacím.
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Technická a aplikační sdělení předních výrobců hmotnostních spektrometrů.
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Cole R. B. (editor). (1997). Electrospray ionization mass spectrometry. Wiley Chichester.
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de Hoffmann E., Stroobant, V. (2002). Mass Spectrometry. Principles and Applications. John Wiley and sons, Chichester.
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Gross, J. H. (2001). Mass Spectrometry, A Textbook. Springer.
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Lee M.S. Mass Spectrometry Handbook, J. Wiley and sons, 2012.
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Původní vědecká sdělení věnovaná novým poznatkům v teorii, novým trendům v instrumentaci a zajímavým aplikacím. Analytical chemistry, Journal of the American society for mass spectrometry, Journal of mass spectrometry, Mass spectrometry review...
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Ubik K. (2000). Fyzikálně-chemické metody, část 2, Hmotnostní spektrometrie. Ústav organické chemie a biochemie AV ČR, Praha.
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Wilkins Ch. L., Trimpin S. (eds.). Ion mobility spectrometry-mass spectrometry: Theory and applications, CRC Press.
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